| Wild Bill Jones | Pharis & Jason Romero | 4:02 | |
| Salt Creek | Clarence White | 2:39 | |
| Down in a Willow Garden | The Kossoy Sisters | 2:34 | |
| The Wife Wrapt in Wether's Skin | Hedy West | 2:32 | |
| Sail Away Lady - 2020 Remaster | Uncle Bunt Stephens | 2:58 | |
| Oh Death | Charley Patton | 2:52 | |
| Living The Right Life Now | Molly O'Day | 2:17 | |
| All the Pretty Little Horses | Alan Lomax | 1:49 | |
| Railroad Bill | Hobart Smith | 2:38 | |
| Come All You Virginia Girl | Foghorn Stringband | 3:07 | |
| Leatherwing Bat | Peggy Seeger | 2:11 | |
| Between the Wars | Molsky's Mountain Drifters | 3:26 | |
| I'll Fly Away | The Kossoy Sisters | 2:32 | |
| Cocaine Done Kill My Baby | Mance Lipscomb | 2:33 | |
| Lazy John | Chance McCoy & The Appalachian String Band | 2:59 | |
| Cuckoo Bird - GemsOnVHS Field Recording | Sam Shackleton, GemsOnVHS | 4:11 | |
| Henry Lee (Original Mix) | Dick Justice | 3:25 | |
| My Old Horse Died | Dock Boggs | 1:48 | |
| Harper | Tom Paxton | 2:52 | |
| The Winding Spring | Frank Fairfield | 1:49 | |
| Milwaukee Blues | Charlie Poole | 3:18 | |
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| Goin' Across the Mountain | Anna & Elizabeth | 2:18 | |
| Ride Old Buck To Water | Nate Leath, Danny Knicely, Tatiana Hargreaves, Mark Schatz, Sarah Jarosz, Eric Robertson | 2:41 | |
| I Don't Want Your Greenback Dollar | The Blue Sky Boys | 2:50 | |
| Mole in the Ground | Bascom Lamar Lunsford | 3:03 | |
| Lost Girl | Foghorn Stringband | 3:06 | |
| Pretty Saro | Rhiannon Giddens | 2:41 | |
| Maybe the Last Time | Reeltime Travellers | 4:56 | |
| Ruby - Are You Mad at Your Man | The Osborne Brothers | 3:01 | |
| On the Banks of the Ohio - From Paper Moon | The Blue Sky Boys | 3:20 | |
| Shelvin Rock | Chance McCoy & The Appalachian String Band | 3:08 | |
| Green Valley Waltz | Nora Brown, Jackson Lynch | 2:49 | |
| Cocaine (Original Mix) | Dick Justice | 3:01 | |
| Here in the Kitchen | Dan Reeder | 1:28 | |
| Little Birdie | Chance McCoy & The Appalachian String Band | 5:03 | |
| Graveyard Blues | Roscoe Holcomb | 3:01 | |
| Snake Baked a Hoecake | Peggy Seeger | 1:06 | |
| That Train That Carried My Girl from Town | The Doc Watson Family | 2:20 | |
| Franklin Roosevelt's Back Again | The New Lost City Ramblers | 2:21 | |
| Chick-A-Li-Lee-Lo | Almeda Riddle | 1:17 | |
| And Am I Born To Die? | The Doc Watson Family | 3:31 | |
| The Faithful Soldier | The Doc Watson Family | 3:31 | |
| Cumberland Gap | Frank Proffitt | 1:25 | |
| Nellie Kane | Hot Rize | 2:58 | |
| Pretty Saro | The Doc Watson Family | 2:17 | |
| Snake Baked A Hoecake | Peggy Seeger | 0:56 | |
| Little Sadie | Doc Watson | 2:01 | |
| Rambling Hobo | The Doc Watson Family | 1:04 | |
| The Drunkard's Hiccups (Jack Of Diamonds) | Dirk Powell, John Hermann, Tim O'Brien | 3:15 | |