| You Can't Get That Stuff No More | Tampa Red | 2:52 | |
| Pussy Cat Blues | Bo Carter | 2:54 | |
| Tain't Nobody's Business If I Do | Frank Stokes | 3:15 | |
| Too Wet to Plow | Johnny Shines | 4:26 | |
| Blind Mamie Forehand | Blind Willie Johnson | 2:41 | |
| Someday Baby Blues | Sleepy John Estes | 2:59 | |
| Going to Move to Alabama | Charley Patton | 3:03 | |
| Sugar Babe | Mance Lipscomb | 2:06 | |
| Dark Road Blues | Willie Lofton | 3:03 | |
| Cool Drink Of Water | Houston Stackhouse | 3:16 | |
| Further Up on the Road | David "Honeyboy" Edwards | 4:20 | |
| Charmin' Betsy | Henry Thomas | 3:01 | |
| Cross And Evil Woman Blues | Rev. Gary Davis | 3:02 | |
| You're Gonna Need Somebody on Your Bond | Blind Willie Johnson | 3:09 | |
| Sugar Baby - 2020 Remaster | Dock Boggs | 2:58 | |
| Cocaine Habit Blues | Memphis Jug Band | 2:48 | |
| Wake Up Jacob - 2020 Remaster | Prince Albert Hunt's Texas Ramblers | 2:55 | |
| Old Original Kokomo Blues | Kokomo Arnold | 2:54 | |
| Anna Lou Blues | Tampa Red | 2:54 | |
| See That My Grave's Kept Clean | Blind Lemon Jefferson | 2:54 | |
| Move This Rope | J.B. Lenoir | 3:34 | |
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| Motherless Children | Blind Willie Johnson | 3:22 | |
| County Farm Blues | Son House | 2:12 | |
| Billy Lyons and Stack O'lee | Furry Lewis | 2:31 | |
| John Hardy Was a Desperate Little Man | The Carter Family | 2:55 | |
| Red River Blues | Henry Thomas | 3:04 | |
| Rock Me Mama | Arthur "Big Boy" Crudup | 2:29 | |
| Down On Penny's Farm | Bentley Boys | 2:48 | |
| John the Revelator | Son House | 2:29 | |
| Some Day Baby | Lonnie Johnson | 3:08 | |
| The Half Ain't Never Been Told / Just Look / Come and See / God Moves on the Water / He Got Better Things for You / The Latter Rain Is Fall | Rev. F.W. McGee, Arizona Dranes, Rev. D.C. Rice, Blind Willie Johnson, Lonnie McIntorsh, Elder Edwards, The Memphis Sanctified Singers | 7:36 | |
| Feelin' Good | J.B. Lenoir | 3:01 | |
| Sweet Honey Hole | Blind Boy Fuller | 2:47 | |
| The Girl I Love, She Got Long Curly Hair | Sleepy John Estes | 2:56 | |
| Evil Devil Woman Blues | Memphis Minnie & Kansas Joe | 3:14 | |
| Minglewood Blues | Cannon's Jug Stompers | 3:40 | |
| Night Time Is the Right Time | Mance Lipscomb | 4:00 | |
| Ride Em on Down | Robert Petway | 2:56 | |
| Mean and Evil Blues | Tampa Red | 2:53 | |
| Take Your Burden to the Lord and Leave It There | Blind Willie Johnson | 2:55 | |
| Wise County Jail | Dock Boggs | 1:52 | |
| Hangman | Spider John Koerner, Dave Ray, Tony Glover | 2:32 | |
| Baby You Gotta Change Your Mind | Blind Boy Fuller | 3:12 | |
| Shake 'Em On Down | Bukka White | 3:00 | |
| Devil Got My Woman | Skip James | 5:15 | |
| Little Boy Blue | Scrapper Blackwell | 3:07 | |
| St. Louis Blues | Lonnie Johnson, Elmer Snowden | 3:05 | |
| John Henry | Furry Lewis | 5:49 | |
| Goin' Away Baby - Single Version | Jimmy Rogers | 2:46 | |