| Poor Ellen Smith | The Kossoy Sisters | 2:28 | |
| Mississippi Heavy Water Blues | Roscoe Holcomb | 2:17 | |
| Sail Away Lady - 2020 Remaster | Uncle Bunt Stephens | 2:58 | |
| The Wild Rippling Water | Alan Lomax | 3:11 | |
| Jesse James | Harry McClintock | 3:28 | |
| Come All You Virginia Girl | Foghorn Stringband | 3:07 | |
| The Old Man Below | The Doc Watson Family | 1:35 | |
| Little Birdie | The Kossoy Sisters | 2:56 | |
| Rattlesnake | Alan Lomax | 1:34 | |
| Minglewood Blues | Cannon's Jug Stompers | 3:40 | |
| Oh Molly Dear | B.F. Shelton | 3:00 | |
| Boil Them Cabage Down | Curly King & The Tennessee Hilltoppers | 1:04 | |
| Banjo Tune | Erik Darling, The Kossoy Sisters, Robin Roberts | 1:42 | |
| Dry Bones - 2020 Remaster | Bascom Lamar Lunsford | 3:00 | |
| Little Birdie | Chance McCoy & The Appalachian String Band | 5:03 | |
| East Tennessee Blues | Bill Monroe and Doc Watson | 2:12 | |
| Elk River Blues | Rockridge Brothers | 2:52 | |
| Goodbye Old Paint | Harry McClintock | 2:49 | |
| Swanno Mountain | Roscoe Holcomb | 3:20 | |
| Down in the Willow Garden (Rose Connolly) | Iron Mountain String Band | 3:16 | |
| Barbara Allen | Bradley Kincaid | 3:25 | |
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| How Can a Poor Man Stand Such Times and Live | The New Lost City Ramblers | 3:35 | |
| John Hardy | Mike Seeger | 2:44 | |
| Cocaine (Original Mix) | Dick Justice | 3:01 | |
| Hungry Hash House | Charlie Poole | 3:25 | |
| Willdwood Flower | Clarence White | 1:25 | |
| Cranberry Rock | Chance McCoy & The Appalachian String Band | 3:24 | |
| Out on the Open West | Frank Fairfield | 3:13 | |
| Foggy Mountain Top | Bill Monroe and Doc Watson | 2:46 | |
| Lost Indian | Foghorn Stringband | 2:57 | |
| Salt Creek | Clarence White | 2:39 | |
| Mistreated Mama Blues | Dock Boggs | 1:54 | |
| Spider In The Window - Instrumental | Blaine Sprouse | 3:02 | |
| Graveyard Blues | Roscoe Holcomb | 3:01 | |
| John Brown | Hobart Smith | 1:51 | |
| Sugar Hill | Mary Z. Cox | 3:33 | |
| Home From The Forest | Tony Rice Unit | 4:19 | |
| Satan's Jeweled Crown | The Louvin Brothers | 2:57 | |
| Poor Ellen Smith | Jim Mills | 2:31 | |
| Soldier's Joy | The Rice Brothers | 1:34 | |
| On My Way Back To The Old Home | The Bluegrass Album Band | 2:32 | |
| Old Joe Clark / June Apple | Tony Trischka | 1:46 | |
| Ginseng Sullivan | Tony Rice Unit | 3:10 | |
| Raymond's Talking Banjo | Raymond Fairchild | 2:09 | |
| Little Sadie | Bill Keith, Jim Rooney | 1:27 | |
| Early, Early In The Spring | The Doc Watson Family | 2:06 | |
| Buck Creek Gal | The Earls Of Leicester | 1:29 | |
| Cumberland Gap | Frank Proffitt | 1:25 | |
| Brilliancy Medley | Eck Robertson | 2:57 | |