| Cause She Thinks She's Made of Candy | Act | 3:26 | |
| Grad Student Shuffle | Chris Fleming | 1:47 | |
| I Want to Be Tall (Broadway Version) | Daniel Ruffing | 2:23 | |
| Toxic Gossip Train (Miranda sings apology) | Tambo | 2:59 | |
| Get Down | SIX, Genesis Lynea | 4:13 | |
| Do Your Boobs Hang Low | Koit | 1:05 | |
| Gay! | Randy Rainbow | 2:44 | |
| Among Us in Real Life | Rebecca Zamolo | 2:39 | |
| Black Chick's Tongue | Drawn Together | 1:40 | |
| Perfect Together | Rosanna Pansino | 2:29 | |
| The Chocolate Chip Cookie Song | Tubby Nugget, The Space Grapes | 1:46 | |
| Music, Music Everywhere! | Jake Gyllenhaal, John Mulaney, The Sack Lunch Bunch | 5:17 | |
| The Wizard of Ahhhs | Todrick Hall, Pentatonix | 6:09 | |
| FU2 | Elton Castee, Lily Lewis | 3:03 | |
| Fluid | Dion Yorkie | 3:03 | |
| Sephora | Jared | 1:29 | |
| Buttholes Are Nothing to Be Laughed at | Laura Clery | 2:12 | |
| A Gay Disney Prince | Thomas Sanders, Jon Cozart | 3:48 | |
| Cinderella vs. Belle (Princess Rap Battle) | Whitney Avalon, Sarah Michelle Gellar | 2:32 | |
| Why Does Nobody (Get How Great I Am?) | Nick Kroll, Jessi Klein, Maya Rudolph, Mark Rivers | 1:25 | |
| Almost Married... | Marygrace Buckner | 3:42 | |
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| Nair Apology (Toxic Gossip Train) | Kevin Leonardo | 2:34 | |
| Don't Fuck Your Coworkers | sailor鄧mel | 1:11 | |
| Make Believe | Joey Graceffa | 2:53 | |
| Skeleton | Mikey Angelo | 2:31 | |
| XO baby | Six Twenty One | 4:05 | |
| The Hannah Poop Song | The Odd Man Who Sings About Poop, Puke and Pee | 2:08 | |
| You, Me and Steve | Garfunkel and Oates | 2:01 | |
| Paparazzi | Emily Alyn Lind | 3:30 | |
| Shut up and Drive | Cheerleading Fierce Factory | 3:33 | |
| We Won't Forget You | N'we Jinan Artists | 4:47 | |
| School Degree | LuxuryPranks | 3:40 | |
| Coffee Shop Bop - Sped Up Version | Sarah Maddack | 1:23 | |
| Look at Spookley | Spookley the Square Pumpkin | 1:21 | |
| Dorothy vs. Alice: Princess Rap Battle | Whitney Avalon | 4:52 | |
| The Hamster Song | The Singing Animal Lover | 1:50 | |
| Three Dots from Stardom | Ben Schwartz | 3:23 | |
| Fart | Sebastian Castro | 3:37 | |
| It's GFORCE | GEN:ZED | 1:33 | |
| I Have 6 Cats | The Gregory Brothers | 1:57 | |
| Politiclash: Donald Trump vs Joe Biden | Jon Cozart | 6:46 | |
| Carl Poppa (feat. Carl G.) | Bad Lip Reading, Carl G. | 4:38 | |
| Makin' Bacon Pancakes (From "Adventure Time") | Dolphin Smiling | 1:14 | |
| Me, You and Steve | Garfunkel and Oates | 1:57 | |
| Sunny Side Up Summer | Bob's Burgers, H. Jon Benjamin, John Roberts, Dan Mintz, Kristen Schaal, Eugene Mirman | 3:57 | |
| Own It | Central Park Cast, Kristen Bell, Tituss Burgess, Kathryn Hahn, Daveed Diggs, Stanley Tucci, Josh Gad, Leslie Odom Jr. | 3:45 | |
| The Comeback Song | Olivia Rodrigo, Madison Hu | 2:31 | |
| Icky Vicky | Nickelodeon | 1:05 | |
| F**k You | The Sockapellas | 1:12 | |
