| Big Road Blues | Tommy Johnson | 3:12 | |
| Worried About My Baby | Sunnyland Slim | 2:41 | |
| Am I Right Or Wrong? | Son House | 1:45 | |
| It's Tight Like That | Tampa Red, Georgia Tom | 3:04 | |
| 34 Blues | Charley Patton | 2:57 | |
| James Alley Blues | Richard "Rabbit" Brown | 3:09 | |
| Sugar Babe | Mance Lipscomb | 2:06 | |
| It's Tight Like That | Tampa Red | 2:49 | |
| Judge Harsh Blues | Furry Lewis | 3:05 | |
| Key To The Highway | Jazz Gillum | 2:43 | |
| Broke and Hungry | Sleepy John Estes, The Tennessee Jug Busters, Mike Bloomfield | 4:32 | |
| You Gonna Take Sick and Die | Boyd Rivers | 3:41 | |
| My Mama Don't Allow Me | Arthur "Big Boy" Crudup | 3:10 | |
| Tears Don't Fall No More | Lonnie Johnson | 3:11 | |
| Rowdy Blues | Kid Bailey | 3:00 | |
| Garter Snake Blues | Victoria Spivey | 3:17 | |
| Don't Ease Me In | Henry Thomas | 2:58 | |
| Take Your Fingers off It | Will Shade, Charlie Burse, Gus Cannon | 2:54 | |
| My Pencil Won't Write No More | Bo Carter | 2:57 | |
| Memphis Rounders Blues | Frank Stokes | 2:51 | |
| Phonograph Blues - Take 1 | Robert Johnson | 2:37 | |
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| Back Home Blues | Big Joe Williams | 4:22 | |
| Banana in Your Fruit Basket | Bo Carter | 3:08 | |
| Minglewood Blues | Cannon's Jug Stompers | 3:40 | |
| West Coast Blues | Blind Blake | 3:08 | |
| Rock Me Mama | Arthur "Big Boy" Crudup | 2:29 | |
| Dead Shrimp Blues | Robert Johnson | 2:32 | |
| When Death Come Creeping in Your Room (Run, Sinner, Run) - Take 1; Remastered Version | Mance Lipscomb | 2:57 | |
| High Water Everywhere, Pt. 1 | Charley Patton | 3:08 | |
| Viola Lee Blues | Cannon's Jug Stompers | 3:10 | |
| On The Road Again | Memphis Jug Band | 2:48 | |
| Watcha Doin'? | Sleepy John Estes, Yank Rachel, Noah Lewis | 3:07 | |
| Hot Fingers | Eddie Lang, Lonnie Johnson | 2:58 | |
| Trouble Mind | Sonny Terry and Brownie McGhee | 3:26 | |
| Cool Drink Of Water | Houston Stackhouse | 3:16 | |
| That Crawlin' Baby Blues | Blind Lemon Jefferson | 2:41 | |
| My Baby Left Me | Arthur "Big Boy" Crudup | 2:30 | |
| Further Up on the Road | David "Honeyboy" Edwards | 4:20 | |
| Ragged And Dirty | William Brown | 4:50 | |
| Chicago Breakdown | Big Maceo | 2:55 | |
| Bad Luck Blues (Alternative Take) | Lightnin' Slim | 2:35 | |
| Everybody Oughta Make a Change | Sleepy John Estes | 2:50 | |
| Stack O' Lee Blues | Ma Rainey | 2:57 | |
| Undertaker Blues | Buddy Moss | 3:14 | |
| Gotta Go Baby (Gotta Let You Go) | Joe Hill Louis | 2:41 | |
| That's All Right - 1950 Single Version | Jimmy Rogers | 2:50 | |
| Eisenhower Blues | J.B. Lenoir | 2:52 | |
| Standing At The Crossroads | Johnny Shines | 2:54 | |
| By The Spoonful | J.D. Short | 3:49 | |