| On the Banks of the Ohio | Bill Monroe | 3:28 | |
| Jake Lake | Jeff Cowell | 2:22 | |
| Rattlesnake | Alan Lomax | 1:34 | |
| Mystery | Jackson C. Frank | 2:34 | |
| Oh babe it ain't no lie | Elizabeth Cotten | 4:43 | |
| Everglades | Dale Hawkins | 2:12 | |
| Chasin' Rainbows | R. Crumb And His Cheap Suit Serenaders | 3:17 | |
| I'll Be Rested (When The Roll Is Called) | Blind Roosevelt Graves | 2:33 | |
| Old Dog Blue (Alternate Take) | Jim Jackson | 3:04 | |
| Coal Creek March | Pete Steele | 1:27 | |
| Nobody's Business | Riley Puckett | 2:40 | |
| Passin' Through | Sam Doores, Riley Downing, The Tumbleweeds | 2:46 | |
| Snake Baked a Hoecake | Peggy Seeger | 1:06 | |
| All the Pretty Little Horses | Alan Lomax | 1:49 | |
| Down This Road | Connie Converse | 1:44 | |
| Die Liebe - Bettina Live | Sebuko, Bettina | 4:41 | |
| In the Heat of the Summer | Phil Ochs | 3:08 | |
| Tennessee Stud | Jimmie Driftwood | 3:21 | |
| Sail Away Lady - 2020 Remaster | Uncle Bunt Stephens | 2:58 | |
| I Must Be Somebody Else You've Known | The International Submarine Band | 2:20 | |
| Bill Bailey | Big Bill Broonzy | 1:57 | |
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| John the Revelator | Blind Willie Johnson | 3:16 | |
| What Will We Do with the Baby O | The Kossoy Sisters | 1:46 | |
| Bring It With You When You Come | Cannon's Jug Stompers | 2:45 | |
| I Am a Man of Constant Sorrow | Roscoe Holcomb | 3:00 | |
| Ommie Wise - 2020 Remaster | G. B. Grayson | 3:13 | |
| Take a Drink on Me | Charlie Poole | 3:17 | |
| The Poor Wayfaring Stranger | Estil C. Ball | 3:14 | |
| Wake Up Jacob - 2020 Remaster | Prince Albert Hunt's Texas Ramblers | 2:55 | |
| Fat Boy | Blaze Foley | 2:37 | |
| Tumble in the Wind (Version 2) | Jackson C. Frank | 3:07 | |
| The Big Rock Candy Mountain | Harry McClintock | 2:15 | |
| Swanno Mountain | Roscoe Holcomb | 3:20 | |
| I've Endured | Ola Belle Reed, Bud Reed, David Reed | 2:52 | |
| When the Cactus Is in Bloom | Jimmie Rodgers | 3:15 | |
| Son of a Gun | Sanford Clark | 2:38 | |
| Mole in the Ground | Bascom Lamar Lunsford | 3:03 | |
| Church, I'm Fully Saved Today | Blind Willie Johnson | 3:06 | |
| Graveyard Blues | Roscoe Holcomb | 3:01 | |
| Junk Sails | Steph Green | 3:20 | |
| Poor Boy Blues | Ramblin' Thomas | 2:29 | |
| Willie Moore | The Kossoy Sisters | 3:05 | |
| Bury Me Beneath the Willow | Clarence White | 1:56 | |
| The Wild Ox Moan | Vera Hall | 1:02 | |
| Snake Baked A Hoecake | Peggy Seeger | 0:56 | |
| Cumberland Gap | Frank Proffitt | 1:25 | |
| Lorene | The Louvin Brothers | 2:03 | |
| Satan's Jeweled Crown | The Louvin Brothers | 2:57 | |
| Soldier's Joy | The Kentucky Colonels | 1:14 | |