| Cluck Old Hen | Frank Proffitt | 2:23 | |
| T.B. Blues (They're at Rest Together) | Hazel Dickens, Alice Gerrard | 3:28 | |
| When I Die | The Doc Watson Family | 2:17 | |
| Cherokee Shuffle | Bill Keith | 3:07 | |
| No Depression in Heaven | The New Lost City Ramblers | 3:00 | |
| Walk Along John To Kansas | Tatiana Hargreaves, Danny Knicely, Mark Schatz, Alex Hargreaves | 3:26 | |
| Roving Gambler | The Country Gentlemen | 3:10 | |
| East Tennessee Blues | Bill Monroe and Doc Watson | 2:12 | |
| Walkin' in My Sleep | Hazel Dickens, Alice Gerrard | 2:10 | |
| Sail Away Lady - 2020 Remaster | Uncle Bunt Stephens | 2:58 | |
| If the River Was Whiskey | Charlie Poole | 3:10 | |
| Cranberry Rock | Chance McCoy & The Appalachian String Band | 3:24 | |
| Little Birdie | The Kossoy Sisters | 2:56 | |
| Wildwood Flower | Kilby Snow | 1:26 | |
| If I Lose, I Don't Care | The North Carolina Ramblers, Charlie Poole | 3:09 | |
| Ain't No Grave | The Crooked Jades | 3:26 | |
| John Brown | Hobart Smith | 1:51 | |
| Way Down The Old Plank Road | Uncle Dave Macon, Sam McGee | 2:58 | |
| Nine Pound Hammer | Jim & Jesse | 2:05 | |
| Mistreated Mama Blues | Dock Boggs | 1:54 | |
| Lee Highway Blues | Hazel Dickens, Alice Gerrard | 1:42 | |
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| Poor Boy | Harry McClintock | 2:14 | |
| What Will We Do with the Baby O | The Kossoy Sisters | 1:46 | |
| Graveyard Blues | Roscoe Holcomb | 3:01 | |
| John Hardy | Frank Proffitt | 2:27 | |
| In the Pines | The Kossoy Sisters | 3:19 | |
| Sand Lake | The Crooked Jades | 2:23 | |
| Cumberland Gap - Remastered | Wade Ward | 1:09 | |
| Ida Red | Stringbean | 1:16 | |
| The Dying Cowboy | Cisco Houston | 3:03 | |
| Ground Hog | The Doc Watson Family | 2:21 | |
| Hills of Mexico | Lonesome Ace Stringband | 4:38 | |
| Berkeley: Horn Trio, Op. 44: III. (d) Variation III. Lento | Lennox Berkeley, Manoug Parikian, Dennis Brain, Colin Horsley | 2:29 | |
| Sam Gass | Harry McClintock | 3:21 | |
| Down in the Willow Garden (Rose Connolly) | Iron Mountain String Band | 3:16 | |
| No Hard Times | Jimmie Rodgers | 3:07 | |
| Willie Taylor | Uncle Earl | 4:11 | |
| Baa Nanny Black Sheep | The Doc Watson Family | 0:56 | |
| Booth Shot Lincoln | Uncle Earl | 2:09 | |
| I'm Bound To Ride | Fiddlin' Arthur Smith, His Dixieliners | 2:19 | |
| Free Little Bird | Cousin Emmy, The New Lost City Ramblers | 1:00 | |
| There Ain't Nobody Gonna Miss Me When I'm Gone | The Kentucky Colonels | 2:51 | |
| Little Maggie | The Doc Watson Family | 2:39 | |
| Black-Eyed Susie | Uncle Earl | 1:49 | |
| June Apple | Wade Ward | 2:27 | |
| Big Sandy River | Kenny Baker | 2:29 | |
| Rock Bottom Blues | The Nashville Bluegrass Band | 2:56 | |
| And Am I Born To Die? | The Doc Watson Family | 3:31 | |
| Dill Pickle Rag | Don Reno, Bill Harrell, The Tennessee Cut-Ups | 1:38 | |