| Sugar Babe | Mance Lipscomb | 2:06 | |
| Rowan County Crew | Dock Boggs | 6:15 | |
| Cocaine Habit Blues | Memphis Jug Band | 2:48 | |
| Devil Sent the Rain Blues | Charley Patton | 3:05 | |
| Church, I'm Fully Saved Today | Blind Willie Johnson | 3:06 | |
| So Different Blues | Mance Lipscomb | 3:05 | |
| The Old lady & the devil | Bill & Belle Reed | 3:06 | |
| Mistreated Mama Blues | Dock Boggs | 1:54 | |
| Keep My Skillet Good and Greasy | Uncle Dave Macon | 2:46 | |
| High Water Everywhere, Pt. 1 (Alternate Take) | Charley Patton | 3:04 | |
| Rag, Mama, Rag | Blind Boy Fuller | 3:03 | |
| Jesse James | Harry McClintock | 3:28 | |
| Jesus Make Up My Dying Bed | Blind Willie Johnson | 3:10 | |
| Trouble in Mind - Version; Take 1; Remastered | Mance Lipscomb | 3:09 | |
| Ruby, Are You Mad At Your Man? | The New Lost City Ramblers, Cousin Emmy | 1:57 | |
| House Carpenter | Peggy Seeger | 4:08 | |
| Cypress Grove Blues | Skip James, Jack Owens | 3:09 | |
| Sail Away Ladies | Uncle Dave Macon and His Fruit-Jar Drinkers | 3:00 | |
| Tom Dooley | Frank Proffitt | 2:44 | |
| Out on the Open West | Frank Fairfield | 3:13 | |
| Moonshiner | Roscoe Holcomb | 2:00 | |
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| That Train That Carried My Girl from Town | The Doc Watson Family | 2:20 | |
| John the Revelator | Blind Willie Johnson | 3:16 | |
| False Hearter Lover Blues | Dock Boggs | 3:22 | |
| Dry Bones - 2020 Remaster | Bascom Lamar Lunsford | 3:00 | |
| Railroad Bill | Etta Baker, Cora Phillips | 1:33 | |
| Red River Blues | Henry Thomas | 3:04 | |
| Poor Black Mattie | R.L. Burnside | 2:06 | |
| Springtime in Uganda | Blaze Foley | 4:41 | |
| Tennessee Stud | Jimmie Driftwood | 3:21 | |
| John Brown's Dream | The New Lost City Ramblers | 1:34 | |
| Big Road Blues | Tommy Johnson | 3:12 | |
| Undone in Sorrow | Ola Belle Reed, Bud Reed, John Coffey | 3:20 | |
| The Butchers Boy - 2020 Remaster | Buell Kazee | 3:04 | |
| New Prisoner's Song | Dock Boggs | 2:57 | |
| Cocaine Done Kill My Baby | Mance Lipscomb | 2:33 | |
| Ida Red | Anna & Elizabeth | 1:58 | |
| T.B. Blues (They're at Rest Together) | Hazel Dickens, Alice Gerrard | 3:28 | |
| I Crave My Pig Meat | Blind Boy Fuller | 2:51 | |
| Bright Sunny South | Dock Boggs | 3:41 | |
| Darling Cory | Roscoe Holcomb | 3:42 | |
| I Am The Light Of This World | Rev. Gary Davis | 3:35 | |
| Fly Around My Pretty Little Miss | Ola Belle Reed | 2:00 | |
| The Great Atomic Power | The Louvin Brothers | 2:42 | |
| Goin' To Sit Down On The Banks Of The River | Rev. Gary Davis | 2:55 | |
| One-Dime Blues | Etta Baker | 2:35 | |
| Little Sadie | Gaither Carlton | 1:00 | |
| Washington D.C. Hospital Center Blues | Skip James | 4:11 | |
| Satan, Your Kingdom Must Come Down | Frank Proffitt | 1:51 | |