| Darkie Uncle Ned | Harry McClintock | 2:01 | |
| Old Dog Blue | Jim Jackson | 2:58 | |
| Hot Fingers | Eddie Lang, Lonnie Johnson | 2:58 | |
| 'Tain't Nobody's Business If I Do, Pt. 1 | Frank Stokes | 3:16 | |
| High Water Everywhere, Pt. 1 (Alternate Take) | Charley Patton | 3:04 | |
| Jesus Lifted Me | Elizabeth Cotten and Brenda Evans | 2:08 | |
| Rattlesnake | Spider John Koerner | 1:37 | |
| Sweet Sweet Thing | The Delmore Brothers | 2:52 | |
| Keep It Clean | Charley Jordan | 2:48 | |
| Tallahatchie River Blues | Mattie Delaney | 2:48 | |
| Little Maggie | Barbara Dane | 2:09 | |
| I Wish I Was a Mole in the Ground | Bascom Lamar Lunsford | 3:22 | |
| Swannanoa Tunnel | Bascom Lamar Lunsford | 3:00 | |
| New River Train (Version 2) | Matokie Slaughter | 2:00 | |
| See That My Grave's Kept Clean | Blind Lemon Jefferson | 2:53 | |
| Old Mountain Dew | The Delmore Brothers | 2:34 | |
| Jaybird March | Etta and Cora Baker | 1:50 | |
| Wake Up Jacob - 2020 Remaster | Prince Albert Hunt's Texas Ramblers | 2:55 | |
| The Banks of the Ohio | The Kossoy Sisters | 3:19 | |
| When I Get Back Home | Michael Hurley | 6:05 | |
| The Unfortunate Rake | A. L. Lloyd and Alf Edwards | 2:59 | |
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| Nobody's Business | Riley Puckett | 2:40 | |
| It's Tight Like That | Tampa Red, Georgia Tom | 3:04 | |
| Engine 143 | The Kossoy Sisters | 4:17 | |
| Way Down The Old Plank Road | Uncle Dave Macon, Sam McGee | 2:58 | |
| Sally Gooden | Eck Robertson | 3:10 | |
| Ragged And Dirty | William Brown | 4:50 | |
| Minglewood Blues | Cannon's Jug Stompers | 3:40 | |
| Come on Boys, Let's Do That Messin' Around | Blind Blake | 2:43 | |
| Black Snake Moan | Blind Lemon Jefferson | 2:50 | |
| The Girl I Love, She Got Long Curly Hair | Sleepy John Estes | 2:56 | |
| Step It up and Go | Blind Boy Fuller | 2:59 | |
| Hungry Hash House | Charlie Poole | 3:25 | |
| Tupelo Blues | Rayna Gellert, Kieran Kane, Philip Jamison, David Mansfield | 2:53 | |
| What Will We Do with the Baby O | The Kossoy Sisters | 1:46 | |
| Lovesick Blues | Emmett Miller | 2:53 | |
| Leatherwing Bat | Peggy Seeger | 2:11 | |
| Ommie Wise - 2020 Remaster | G. B. Grayson | 3:13 | |
| Greasy Greens | Pink Anderson | 4:38 | |
| Crawdad Hole | Jessie Mae Hemphill | 2:07 | |
| Indian War Whoop | Floyd Ming & His Pep Steppers | 3:09 | |
| Ground Hog Blues - Single Version | John Lee Hooker | 2:55 | |
| House Of The Risin' Sun - Rising Sun Blues | Clarence Ashley | 2:39 | |
| Sinner Man | The Weavers | 2:22 | |
| My Epitaph | Ola Belle Reed | 3:10 | |
| A Conversation With Death | Lloyd Chandler | 4:10 | |
| Someday Soon | Ian & Sylvia | 2:16 | |
| I've Endured | Ola Belle Reed | 2:34 | |
| Are You Afraid To Die | The Louvin Brothers | 2:35 | |