| Motherless Children | Blind Willie Johnson | 3:22 | |
| Down in Mississippi | J.B. Lenoir | 3:16 | |
| Hard Time Killin' Floor Blues | Skip James | 2:49 | |
| Billy Lyons and Stack O'lee | Furry Lewis | 2:31 | |
| Back Door Blues | Kokomo Arnold | 3:25 | |
| Bob McKinney | Henry Thomas | 2:53 | |
| Papa's Got Your Water On | Memphis Jug Band | 2:32 | |
| Special Streamline | Bukka White | 2:47 | |
| Sweet Honey Hole | Blind Boy Fuller | 2:47 | |
| Some Day Baby | Lonnie Johnson | 3:08 | |
| Catfish Blues | Corey Harris | 2:40 | |
| Mean Old Frisco Blues - Remastered 2003 | Arthur "Big Boy" Crudup | 2:35 | |
| On The Road Again | Memphis Jug Band | 2:48 | |
| Big Road Blues | Tommy Johnson | 3:12 | |
| Searchin' the Desert for the Blues | Blind Willie McTell | 3:09 | |
| Tain't Nobody's Business If I Do | Frank Stokes | 3:15 | |
| Jesus Make Up My Dying Bed | Blind Willie Johnson | 3:10 | |
| Buck Dance - Version; Instrumental; Remastered | Mance Lipscomb | 1:33 | |
| Milk Cow Blues | Sleepy John Estes | 3:02 | |
| Step It up and Go | Blind Boy Fuller | 2:59 | |
| I'm A Bad Luck Woman | Memphis Minnie | 3:00 | |
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| Everybody Ought to Treat a Stranger Right | Blind Willie Johnson, Willie B. Richardson | 3:05 | |
| Mashing That Thing | Bo Carter | 2:40 | |
| Catfish Blues | David "Honeyboy" Edwards | 3:30 | |
| On the Road Again | Memphis Jug Band | 2:50 | |
| Let Your Light Shine on Me | Blind Willie Johnson | 3:08 | |
| 34 Blues | Charley Patton | 2:57 | |
| Whoopee Blues | King Solomon Hill | 3:11 | |
| Trouble Mind | Sonny Terry and Brownie McGhee | 3:26 | |
| Black Snake Moan | Blind Lemon Jefferson | 2:50 | |
| When Death Come Creeping in Your Room (Run, Sinner, Run) - Take 1; Remastered Version | Mance Lipscomb | 2:57 | |
| He's In The Jail House Now | Memphis Jug Band | 3:12 | |
| Leavin' Here | J.B. Lenoir | 1:38 | |
| Mean Ol' Frisco | Arthur "Big Boy" Crudup | 2:37 | |
| Stack-O-Lee | Champion Jack Dupree | 3:56 | |
| Friar's Point Blues | Robert Nighthawk | 2:54 | |
| Sweeter as the Years Roll By | Blind Willie Johnson | 2:46 | |
| Pinetop's Boogie Woogie - Remaster | Pinetop Smith | 3:24 | |
| When the Train Comes Along | Henry Thomas | 2:56 | |
| Minglewood Blues | Cannon's Jug Stompers | 3:40 | |
| Standing At The Crossroads | Johnny Shines | 2:54 | |
| White Lightinin' | Furry Lewis | 3:26 | |
| Mother Earth | Memphis Slim | 2:39 | |
| Mean Old World | T-Bone Walker, Freddie Slack | 2:54 | |
| This Train | Sister Rosetta Tharpe | 2:49 | |
| Rollin' And Tumblin' | Big Joe Williams | 2:36 | |
| John Henry | Furry Lewis | 5:49 | |
| Goin' To Sit Down On The Banks Of The River | Rev. Gary Davis | 2:55 | |
| Preachin' Blues | Son House | 5:16 | |