| The Lone Star Trail | Ken Maynard | 3:12 | |
| Mother's Last Word To Her Son | Washington Phillips | 2:52 | |
| Free at Last | Dock Reed, Vera Hall | 1:41 | |
| Grinnin' in Your Face | Son House | 2:06 | |
| I Wish I Was a Mole in the Ground | Bascom Lamar Lunsford | 3:22 | |
| Old Dollar Mamie | Alan Lomax | 3:24 | |
| Railroad Bill - Remastered | Hobart Smith, Alan Lomax | 2:42 | |
| Cocaine Done Kill My Baby | Mance Lipscomb | 2:33 | |
| Lucky Holler | Ed Lewis | 2:20 | |
| I Got Mine | Frank Stokes | 3:06 | |
| If I Had My Way I'd Tear the Building Down | Blind Willie Johnson | 3:07 | |
| Bob McKinney | Henry Thomas | 2:53 | |
| When I Was a Young Girl | Barbara Dane | 3:43 | |
| Prettiest Train | Alan Lomax | 3:43 | |
| Levee Camp Blues | Son House | 3:45 | |
| Minglewood Blues | Cannon's Jug Stompers | 3:40 | |
| You Gonna Take Sick and Die | Boyd Rivers | 3:41 | |
| Church, I'm Fully Saved Today | Blind Willie Johnson | 3:06 | |
| Parchman Farm Blues | Bukka White | 2:42 | |
| When the Train Comes Along | Henry Thomas | 2:56 | |
| Omie Wise | Doug and Jack Wallin | 3:05 | |
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| Gimme a Pigfoot and a Bottle of Beer | Bessie Smith | 3:27 | |
| Pick Poor Robin Clean | Geeshie Wiley | 3:14 | |
| Keep It Clean | Charley Jordan | 2:48 | |
| High Water Everywhere, Pt. 1 (Alternate Take) | Charley Patton | 3:04 | |
| Step It up and Go | Blind Boy Fuller | 2:59 | |
| Little Maggie | Barbara Dane | 2:09 | |
| Blow, Gabriel | The McIntosh County Shouters | 3:37 | |
| Looking for a Sweetie | Lonnie Johnson | 3:48 | |
| Jail-House Blues | Bessie Smith | 3:13 | |
| Diamond Joe | Bessie Jones, Alan Lomax | 1:19 | |
| Canned Heat Blues | Tommy Johnson | 3:39 | |
| Oh Death | Charley Patton | 2:52 | |
| Wake Up Jacob - 2020 Remaster | Prince Albert Hunt's Texas Ramblers | 2:55 | |
| Snake Baked a Hoecake | Peggy Seeger | 1:06 | |
| Rocky Road | Alabama Sacred Harp Singers | 2:46 | |
| On the Road Again | Memphis Jug Band | 2:50 | |
| Dark Night Blues | Blind Willie McTell | 2:56 | |
| Moan, You Moaners | Bessie Smith | 3:11 | |
| Down On Penny's Farm | Bentley Boys | 2:48 | |
| On That Rock | Viola James | 2:56 | |
| Long Road to Travel | Lonnie Johnson | 2:22 | |
| Señorita Panchita | Neville Marcano (The Growling Tiger), Alan Lomax | 3:07 | |
| Sugar Babe | Mance Lipscomb | 2:06 | |
| What Is The Soul Of Man? | Dock Reed, Henry Reed, Vera Hall | 1:21 | |
| Judge Boushay Blues | Furry Lewis | 3:35 | |
| Another Man Done Gone - Hitchild Remix | Adam Fearn, Vera Hall, Hitchild | 6:28 | |
| Leaving Blues | Davy Graham | 2:54 | |
| Pure Religion | Rev. Gary Davis | 2:57 | |
