| Engine 143 | The Kossoy Sisters | 4:17 | |
| Oh The Wind And Rain | Jody Stecher | 3:49 | |
| Wake Up Jacob - 2020 Remaster | Prince Albert Hunt's Texas Ramblers | 2:55 | |
| Cindy | Frank Proffitt | 2:16 | |
| Bury Me Beneath the Willow | Clarence White | 1:56 | |
| Wildwood Flower | Kilby Snow | 1:26 | |
| The Wind Is Blowin' | Johnny Shines | 3:40 | |
| Ain't You Sorry | Mance Lipscomb | 2:20 | |
| Black Mountain Blues (Rag) | Clarence White | 1:37 | |
| Stewball | Willie Watson | 3:47 | |
| Ground Hog | The Doc Watson Family | 2:21 | |
| Sweet Sweet Thing | The Delmore Brothers | 2:52 | |
| Uncle Jim's Rebel Soldier | Harry McClintock | 2:35 | |
| Willie Moore | The Kossoy Sisters | 3:05 | |
| Go Dig My Grave | Jean Ritchie and Doc Watson | 3:50 | |
| Hungry Hash House | Charlie Poole | 3:25 | |
| Omie Wise | Doug and Jack Wallin | 3:05 | |
| The Banks of the Arkansas / Wave the Ocean | Neil Morris | 1:58 | |
| Mother's Advice | Dock Boggs | 3:42 | |
| Black Snake Moan | Blind Lemon Jefferson | 2:50 | |
| Trifling Woman | Frank Proffitt | 2:17 | |
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| Bluegrass Banjo | Erik Darling, The Kossoy Sisters, Robin Roberts | 1:41 | |
| Springtime of Life | Ola Belle Reed, Bud Reed, David Reed | 4:22 | |
| Cocaine Habit Blues | Memphis Jug Band | 2:48 | |
| Call Me A Dog When I'm Gone | Frank Fairfield | 2:24 | |
| Catfish Blues | Robert Petway | 2:55 | |
| Greasy Coat | Chance McCoy & The Appalachian String Band | 3:02 | |
| Beaver Dam Road | Frank Proffitt | 1:26 | |
| Tell It To Me | Grant Brothers & Their Music | 2:56 | |
| Sugar Babe | Mance Lipscomb | 2:06 | |
| Dry Bones - 2020 Remaster | Bascom Lamar Lunsford | 3:00 | |
| Old Country Stomp | Henry Thomas | 2:51 | |
| Prodigal Son | Dock Boggs | 3:57 | |
| Sam Gass | Harry McClintock | 3:21 | |
| Arkansas Traveler | Jimmie Driftwood | 2:07 | |
| Nine Pound Hammer | Frank Fairfield | 3:57 | |
| Railroad Bill | Hobart Smith | 2:38 | |
| Green Mountain Hop | Reno & Smiley | 2:14 | |
| Sail Away Lady - 2020 Remaster | Uncle Bunt Stephens | 2:58 | |
| Pretty Polly | Dock Boggs | 2:55 | |
| Reuben's Train | Foghorn Stringband | 3:34 | |
| Goin' Down This Road | Big Bill Broonzy | 2:22 | |
| Satan, Your Kingdom Must Come Down | Frank Proffitt | 2:11 | |
| I'm Troubled | The Doc Watson Family | 2:42 | |
| Ragged But Right | Riley Puckett | 2:57 | |
| Ola Belle's Blues | Ola Belle Reed, Kevin Roth | 2:22 | |
| Baby, It Must Be Love | Blind Willie McTell | 1:44 | |
| I Am The Light Of This World | Rev. Gary Davis | 3:35 | |
| Listen To The Mockingbird | The Kentucky Colonels | 1:13 | |