| Reconstructed Rebel Soldier | Harry McClintock | 2:41 | |
| Wild Bill Jones | Charlie Parr | 2:56 | |
| Cross Road Blues - Take 1 | Robert Johnson | 2:37 | |
| Sic 'Em Dogs On | Bukka White | 2:27 | |
| Little Birdie | The Kossoy Sisters | 2:56 | |
| Single Girl | Roscoe Holcomb | 3:02 | |
| The Butchers Boy - 2020 Remaster | Buell Kazee | 3:04 | |
| Barbara Allen | Bradley Kincaid | 3:25 | |
| Sail Away Lady - 2020 Remaster | Uncle Bunt Stephens | 2:58 | |
| Ramblin' Blues | Charlie Poole | 3:07 | |
| Country Blues | Dock Boggs | 3:52 | |
| Ozark Bill | Jimmy Driftwood | 2:46 | |
| Cindy | Frank Proffitt | 2:16 | |
| Snake Baked a Hoecake | Peggy Seeger | 1:06 | |
| Kokomo Blues | Scrapper Blackwell | 3:06 | |
| Swanno Mountain | Roscoe Holcomb | 3:20 | |
| The Road Is Rough and Rocky | Archie Edwards | 3:27 | |
| Billy Lyons and Stack O'lee | Furry Lewis | 2:31 | |
| Banjo Tune | Erik Darling, The Kossoy Sisters, Robin Roberts | 1:42 | |
| My Epitaph | Ola Belle Reed, Bud Reed, David Reed | 3:16 | |
| Fishing Blues | Henry Thomas | 2:42 | |
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| Carolina Breakdown | Etta Baker | 3:11 | |
| Little Maggie | Roscoe Holcomb | 3:02 | |
| Levee Camp Blues | Son House | 3:45 | |
| Foggy Mountain Top | Ola Belle Reed, Bud Reed, Kevin Roth | 2:32 | |
| Say No to the Devil | Rev. Gary Davis | 3:20 | |
| The L and N Don't Stop Here Anymore - Remastered | Jean Ritchie | 3:14 | |
| Suicide Blues | Stringbean | 2:26 | |
| Nobody's Business | Riley Puckett | 2:40 | |
| If the River Was Whiskey | Charlie Poole | 3:10 | |
| Barbara Allen | Bradley Kincaid | 2:32 | |
| A Spoonful Blues | Charley Patton | 3:12 | |
| I Got Mine | Frank Stokes | 3:06 | |
| I'll Be Rested (When The Roll Is Called) | Blind Roosevelt Graves | 2:33 | |
| Moonshiner | Roscoe Holcomb | 2:00 | |
| Talking Banjo | Buzz Busby | 1:39 | |
| Shake 'Em On Down | Bukka White | 3:37 | |
| The House Carpenter | Jean Ritchie | 4:22 | |
| Ain't You Sorry | Mance Lipscomb | 2:20 | |
| Big Road Blues | Tommy Johnson | 3:12 | |
| Church, I'm Fully Saved Today | Blind Willie Johnson | 3:06 | |
| Bonaparte's Retreat | Ola Belle Reed, Kevin Roth, Bud Reed | 2:49 | |
| Let Me Ride in Your Big Cadillac | Blaze Foley | 2:31 | |
| I'm Bound To Ride | Fiddlin' Arthur Smith, His Dixieliners | 2:19 | |
| Wayfaring Pilgrim | Ola Belle Reed | 4:33 | |
| Sammie, Where Have You Been So Long | Dock Boggs | 3:05 | |
| New Prisoner's Song | Dock Boggs | 2:53 | |
| (Bury Me Under The) Weeping Willow | Mother Maybelle Carter | 2:40 | |
| Pure Religion | Rev. Gary Davis | 2:57 | |