| Mother's Advice | Dock Boggs | 3:42 | |
| Jesse James | Harry McClintock | 3:28 | |
| I Am a Man of Constant Sorrow | Roscoe Holcomb | 3:00 | |
| Last Cigarette | Paul Clayton | 2:03 | |
| Freeborn Man | Tony Rice | 2:50 | |
| Loving Nancy | Dock Boggs | 2:30 | |
| Fishing Blues | Henry Thomas | 2:42 | |
| Cocaine Habit Blues | Memphis Jug Band | 2:48 | |
| Lonesome Yodel Blues | The Delmore Brothers | 2:37 | |
| Honey Babe I'm Bound To Ride | Stringbean | 1:56 | |
| Little Black Train | Dock Boggs | 2:55 | |
| Church, I'm Fully Saved Today | Blind Willie Johnson | 3:06 | |
| White House Blues | Charlie Poole | 3:31 | |
| I Saw the Light | Ola Belle Reed, John Coffey, Bud Reed, David Reed, Alex Campbell | 4:56 | |
| Way Down The Old Plank Road | Uncle Dave Macon, Sam McGee | 2:58 | |
| Satan, Your Kingdom Must Come Down | Frank Proffitt | 2:11 | |
| Fat Boy | Blaze Foley | 2:37 | |
| Graveyard Blues | Roscoe Holcomb | 3:01 | |
| Railroad Blues | Sam McGee | 2:48 | |
| Bury Me Beneath the Willow | Clarence White | 1:56 | |
| If the River was Whiskey | Frank Fairfield | 2:05 | |
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| Kassie Jones (Casey Jones) | Furry Lewis | 2:58 | |
| The Wind Is Blowin' | Johnny Shines | 3:40 | |
| Long Haired Preacher (Preacher and the Slave) | Harry McClintock | 3:18 | |
| Little Delia | Blind Willie McTell | 3:02 | |
| Down in the Willow Garden (Rose Connolly) | Iron Mountain String Band | 3:16 | |
| Death Letter Blues | Son House | 4:19 | |
| Tennessee Stud | Jimmie Driftwood | 3:21 | |
| I'll Never Get Drunk No More | Frank Proffitt | 2:34 | |
| Frosty Morn | The Doc Watson Family | 1:41 | |
| Pick Poor Robin Clean | Geeshie Wiley | 3:14 | |
| Ola Belle's Blues | Ola Belle Reed, Kevin Roth | 2:22 | |
| Railroad Bill | Hobart Smith | 2:38 | |
| Railroad Bill | Etta Baker, Cora Phillips | 1:33 | |
| On The Road Again | Memphis Jug Band | 2:48 | |
| If the River Was Whiskey | Charlie Poole | 3:10 | |
| Chew Tobacco Rag | Woody Pines | 2:04 | |
| Bootlegger's Story | The New Lost City Ramblers | 2:42 | |
| The Butcher Boy | Peggy Seeger | 2:20 | |
| Sugar Hill | Iron Mountain String Band | 3:39 | |
| The Banks of the Ohio | The Kossoy Sisters | 3:19 | |
| Turn Your Radio On (I) | John Hartford | 1:22 | |
| Omie Wise | Doug and Jack Wallin | 3:05 | |
| Sittin' On Top of the World | Sam Chatmon | 3:00 | |
| The Banks of the Arkansas / Wave the Ocean | Neil Morris | 1:58 | |
| Pretty Polly | Jean Ritchie and Doc Watson | 2:25 | |
| Dark Holler | Clarence "Tom" Ashley | 2:58 | |
| Skippin' In The Mississippi Dew | John Hartford | 3:01 | |
| Kill It Kid - Bonus Track | Blind Willie McTell | 2:31 | |