| Springtime of Life | Ola Belle Reed, Bud Reed, David Reed | 4:22 | |
| White House Blues | Charlie Poole | 3:31 | |
| That Train That Carried My Girl from Town | The Doc Watson Family | 2:20 | |
| I Get My Whiskey From Rockingham | Earl Johnson | 3:01 | |
| Bowling Green | The New Lost City Ramblers, Cousin Emmy | 1:50 | |
| Hangman | Jean Ritchie | 2:02 | |
| Going to Germany | Cannon's Jug Stompers | 2:31 | |
| Omie Wise | Doug and Jack Wallin | 3:05 | |
| I Wish I Was a Mole in the Ground | Bascom Lamar Lunsford | 3:22 | |
| Down the Road | The Doc Watson Family | 1:41 | |
| Bowling Green | The Kossoy Sisters | 2:13 | |
| Jesse James | Harry McClintock | 3:28 | |
| Jesse James | The Country Gentlemen | 2:39 | |
| John Hardy | Mike Seeger | 2:44 | |
| Little Black Train | Dock Boggs | 2:55 | |
| The House Carpenter - 2020 Remaster | Clarence Ashley | 3:18 | |
| Call Me A Dog When I'm Gone | Frank Fairfield | 2:24 | |
| Kassie Jones, Pt. 2 | Furry Lewis | 3:04 | |
| Banjo Tune | Erik Darling, The Kossoy Sisters, Robin Roberts | 1:42 | |
| Skin and Bones | Jean Ritchie | 1:07 | |
| The Butchers Boy - 2020 Remaster | Buell Kazee | 3:04 | |
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| Jesus Make Up My Dying Bed | Blind Willie Johnson | 3:10 | |
| I'll Be Rested (When The Roll Is Called) | Blind Roosevelt Graves | 2:33 | |
| When the Train Comes Along | Henry Thomas | 2:56 | |
| Oh The Wind And Rain | Jody Stecher | 3:49 | |
| Poor Boy | Harry McClintock | 2:14 | |
| Rabbit Chase | The New Lost City Ramblers | 2:36 | |
| God Moves On the Water | Blind Willie Johnson | 2:59 | |
| Worried Blues | Mike Seeger | 3:29 | |
| Engine 143 | The Kossoy Sisters | 4:17 | |
| Ramblin' Blues | Charlie Poole | 3:07 | |
| Sam Gass | Harry McClintock | 3:21 | |
| Sally Gooden | Eck Robertson | 3:10 | |
| Ola Belle's Blues | Ola Belle Reed, Kevin Roth | 2:22 | |
| Kentucky Bootlegger | The New Lost City Ramblers | 2:09 | |
| Reuben's Train | Foghorn Stringband | 3:34 | |
| So Different Blues | Mance Lipscomb | 3:05 | |
| Lord I Just Can't Keep From Crying | Blind Willie Johnson | 3:00 | |
| Ozark Bill | Jimmy Driftwood | 2:46 | |
| Bad Luck Blues | Blind Lemon Jefferson | 2:52 | |
| Wayfaring Pilgrim | Ola Belle Reed | 4:33 | |
| Hard Time Killing Floor Blues | Skip James | 3:23 | |
| In The Pines | The Kentucky Colonels | 2:24 | |
| Dill Pickle Rag | Don Reno, Bill Harrell, The Tennessee Cut-Ups | 1:38 | |
| Blue Diamond Mines | The Johnson Mountain Boys | 5:11 | |
| Pure Religion | Rev. Gary Davis | 2:57 | |
| Listen To The Mockingbird | The Kentucky Colonels | 1:13 | |
| One-Dime Blues | Etta Baker | 2:35 | |
| House Of The Risin' Sun - Rising Sun Blues | Clarence Ashley | 2:39 | |