| Guitar Rag | Sylvester Weaver | 3:06 | |
| Red River Blues | Henry Thomas | 3:04 | |
| Mashing That Thing | Bo Carter | 2:40 | |
| Cross And Evil Woman Blues | Rev. Gary Davis | 3:02 | |
| Mississippi Road | J.B. Lenoir | 3:00 | |
| Pinetop's Boogie Woogie - Remaster | Pinetop Smith | 3:24 | |
| When the Train Comes Along | Henry Thomas | 2:56 | |
| Sitting On Top Of The World | Mississippi Sheiks | 3:12 | |
| Match Box Blues | Blind Lemon Jefferson | 3:03 | |
| Keep My Skillet Good and Greasy | Uncle Dave Macon | 2:32 | |
| I Found a New Love | Magic Sam, Earnest Johnson, Mighty Joe Young, Odie Payne, Jr., Stockholm Slim | 4:08 | |
| My Pencil Won't Write No More | Bo Carter | 2:57 | |
| Prayer of Death, Pt. 2 | Charley Patton | 2:52 | |
| Baby You Gotta Change Your Mind | Blind Boy Fuller | 3:12 | |
| Baby Please Don't Go | Big Joe Williams | 2:44 | |
| Walkin' Blues (Original Mix) | Son House | 2:52 | |
| John Henry | Henry Thomas | 2:43 | |
| Further Up on the Road | David "Honeyboy" Edwards | 4:20 | |
| Jersey Bull Blues | Charley Patton | 3:08 | |
| Blues For The Lowland | Sonny Terry and Brownie McGhee | 4:46 | |
| Pussy Cat Blues | Bo Carter | 2:54 | |
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| Bob McKinney | Henry Thomas | 2:53 | |
| Floating Bridge | Sleepy John Estes | 3:12 | |
| Old Dog Blue | Jim Jackson | 2:58 | |
| Mississippi Bottom Blues | Kid Bailey | 2:49 | |
| Alabama March | J.B. Lenoir | 2:53 | |
| Sugar Baby - 2020 Remaster | Dock Boggs | 2:58 | |
| Open Your Book, Your Daddy Got to Read With You | Sam Chatmon | 1:46 | |
| Levee Camp Blues | Son House | 3:45 | |
| Rope Stretching Blues | Blind Blake | 3:02 | |
| Beefsteak Blues | James "Son" Thomas | 2:55 | |
| When Can I Change My Clothes? | Bukka White | 2:59 | |
| My Black Mama, Pt. 1 | Son House | 3:05 | |
| Oh Death | Charley Patton | 2:52 | |
| Railroad Blues | Sam McGee | 2:48 | |
| That's All Right | Arthur "Big Boy" Crudup | 2:53 | |
| Butter And Egg Man Blues | Papa Charlie Jackson | 2:59 | |
| Green River Blues | Charley Patton | 3:11 | |
| She-Wolf | Jessie Mae Hemphill | 4:41 | |
| Ice Cream Man | John Brim | 2:44 | |
| Samson And Delilah | Rev. Gary Davis | 4:03 | |
| Mystery Train - Remastered 2022 | Little Junior Parker, The Blue Flames | 2:24 | |
| Roberta | Furry Lewis | 2:34 | |
| Tryin' To Get Home | Rev. Gary Davis | 3:47 | |
| Story Of My Life | Guitar Slim | 3:00 | |
| When I First Left Home | Big Joe Williams | 3:09 | |
| Great Change Since I Been Born | Rev. Gary Davis | 4:03 | |
| Mother Earth | Memphis Slim | 2:39 | |
| Goin' Down Swingin' | Homesick James | 3:46 | |