| Screamin' and Hollerin' the Blues | Charley Patton | 3:05 | |
| Bright Sunny South | Dock Boggs | 3:41 | |
| Rag, Mama, Rag | Blind Boy Fuller | 3:03 | |
| That Certain Female | Charlie Feathers | 3:01 | |
| You Better Run | Junior Kimbrough | 7:33 | |
| Sure 'Nuff 'n' Yes, I Do | Captain Beefheart & His Magic Band | 2:14 | |
| Spoonful | Willie Dixon | 4:55 | |
| Too Wet to Plow | Johnny Shines | 4:26 | |
| Go to Sleep Little Baby | Bessie Jones | 1:14 | |
| 'Tain't Nobody's Business If I Do, Pt. 1 | Frank Stokes | 3:16 | |
| The Half Ain't Never Been Told / Just Look / Come and See / God Moves on the Water / He Got Better Things for You / The Latter Rain Is Fall | Rev. F.W. McGee, Arizona Dranes, Rev. D.C. Rice, Blind Willie Johnson, Lonnie McIntorsh, Elder Edwards, The Memphis Sanctified Singers | 7:36 | |
| Henry Lee (Original Mix) | Dick Justice | 3:25 | |
| Do the Romp | Junior Kimbrough | 3:57 | |
| Minglewood Blues | Cannon's Jug Stompers | 3:40 | |
| Fishing Blues | Henry Thomas | 2:42 | |
| Some Day Baby | Lonnie Johnson | 3:08 | |
| She Left Me a Mule to Ride | Big Joe Williams | 2:35 | |
| Rollin' and Tumblin' | Elmore James | 2:32 | |
| Meet Me in the City | Junior Kimbrough | 3:11 | |
| I'll Fly Away | The Kossoy Sisters | 2:32 | |
| Cross Road Blues - Take 2 | Robert Johnson | 2:27 | |
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| Drop Down Mama | Sleepy John Estes | 3:11 | |
| I'm Gonna Run to the City of Refuge | Blind Willie Johnson | 3:22 | |
| Tumbling Tumbleweeds | Sons of the Pioneers | 2:42 | |
| Mississippi Heavy Water Blues | Barbecue Bob | 3:09 | |
| My Name Is Carnival | Jackson C. Frank | 3:44 | |
| Nobody Knows You When Your Down and Out | Josh White | 3:00 | |
| When The Levee Breaks | Memphis Minnie & Kansas Joe | 3:08 | |
| Step by Step | Jesse Winchester | 2:57 | |
| Step It up and Go | Blind Boy Fuller | 2:59 | |
| Early In The Mornin' | C.W. Stoneking | 3:36 | |
| Meet Me in the City | Junior Kimbrough | 2:31 | |
| It Hurts Me Too | Elmore James | 3:20 | |
| Preachin' Blues | Son House | 5:43 | |
| Junker's Blues | Champion Jack Dupree | 3:12 | |
| Lazybones | Leon Redbone | 3:06 | |
| Banana in Your Fruit Basket | Bo Carter | 3:08 | |
| Raise the Window High | Lonnie Johnson | 2:39 | |
| Backlash Blues | Nina Simone | 2:28 | |
| Where Did You Sleep Last Night | Lead Belly | 3:01 | |
| Take Your Stand | Blind Willie Johnson | 3:00 | |
| Lonesome Valley | The Fairfield Four | 4:07 | |
| Sorry You're Sick | Ted Hawkins | 2:10 | |
| Eisenhower Blues | J.B. Lenoir | 2:52 | |
| Standing At The Crossroads | Johnny Shines | 2:54 | |
| Greasy Greens | Pink Anderson | 4:38 | |
| Haunted House | Lonnie Johnson, Elmer Snowden | 5:00 | |
| Graveyard Blues | Robert Pete Williams | 2:46 | |
| 900 Miles | Terry Callier | 5:07 | |