| Bukka Jitterburg Swing | Bukka White | 2:35 | |
| Hungry Hash House | Charlie Poole | 3:25 | |
| Railroad Bill | Etta Baker, Cora Phillips | 1:33 | |
| Bile Them Cabbage Down | David Johnson | 2:39 | |
| Ragged But Right | Riley Puckett | 2:57 | |
| Lord I Just Can't Keep From Crying | Blind Willie Johnson | 3:00 | |
| Run Little Rabbit Run | Stringbean | 1:39 | |
| Talking Banjo | Buzz Busby | 1:39 | |
| Comin' Round the Mountain | Uncle Dave Macon, Sam McGee | 3:07 | |
| Bandit Cole Younger | Edward L. Crain | 2:53 | |
| Little Birdie | The Kossoy Sisters | 2:56 | |
| God Moves On the Water | Blind Willie Johnson | 2:59 | |
| Wake Up Jacob - 2020 Remaster | Prince Albert Hunt's Texas Ramblers | 2:55 | |
| Long Haired Preacher (Preacher and the Slave) | Harry McClintock | 3:18 | |
| Swannanoa Tunnel | Bascom Lamar Lunsford | 3:00 | |
| Carolina Breakdown | Etta Baker | 3:11 | |
| Statesboro Blues | Blind Willie McTell | 2:32 | |
| Bob McKinney | Henry Thomas | 2:53 | |
| Mayor of Alburg | Michael Hurley | 1:55 | |
| I Am a Man of Constant Sorrow | Roscoe Holcomb | 3:00 | |
| The Devil's Dream | Blind Boy Paxton | 3:25 | |
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| When Things Go Wrong (It Hurts Me Too) | Big Bill Broonzy | 3:00 | |
| Hello Stranger | Any Old Time String Band | 2:18 | |
| Bugger Burns | Spider John Koerner, Dave Ray, Tony Glover | 1:40 | |
| Milwaukee Blues | Charlie Poole | 3:18 | |
| Blaze Foley's 113th Wet Dream | Blaze Foley | 3:05 | |
| Cigarette Blues | Bo Carter | 3:18 | |
| Bluegrass Banjo | Erik Darling, The Kossoy Sisters, Robin Roberts | 1:41 | |
| Undone in Sorrow | Ola Belle Reed, Bud Reed, John Coffey | 3:20 | |
| Across the Rocky Mountain | Roscoe Holcomb | 5:22 | |
| Livin' in the Woods in a Tree | Blaze Foley | 2:09 | |
| John Henry | Henry Thomas | 2:43 | |
| Let Your Light Shine on Me | Blind Willie Johnson | 3:08 | |
| Cripple Creek | Stringbean | 1:29 | |
| Polly Vaughn | The Dillards | 2:50 | |
| When I Die | The Doc Watson Family | 2:17 | |
| Oh Death | Charley Patton | 2:52 | |
| Sugar Hill | Iron Mountain String Band | 3:39 | |
| Sam Gass | Harry McClintock | 3:21 | |
| Out on the Open West | Frank Fairfield | 3:13 | |
| Rowan County Crew | Dock Boggs | 6:15 | |
| I Got Mine | Frank Stokes | 3:06 | |
| Forkey Deer | Sidna Myers | 2:05 | |
| One-Dime Blues | Etta Baker | 2:35 | |
| Someday Soon | Ian & Sylvia | 2:16 | |
| Soldier's Joy | The Kentucky Colonels | 1:14 | |
| Over Yonder In The Graveyard | Ola Belle Reed | 3:06 | |
| Old Blue | Furry Lewis | 3:00 | |
| Ground Hog Blues - Single Version | John Lee Hooker | 2:55 | |