| Calvary | Dock Boggs | 3:33 | |
| Keep My Skillet Good and Greasy | Uncle Dave Macon | 2:46 | |
| Cocaine (Original Mix) | Dick Justice | 3:01 | |
| Wild Bill Jones | Frank Proffitt | 3:27 | |
| The Cuckoo Bird | The Doc Watson Family | 3:03 | |
| Church, I'm Fully Saved Today | Blind Willie Johnson | 3:06 | |
| Sam Gass | Harry McClintock | 3:21 | |
| Little Maggie | Iron Mountain String Band | 3:10 | |
| Good Morning Judge | Furry Lewis | 5:31 | |
| Keep My Skillet Good and Greasy | Uncle Dave Macon | 2:32 | |
| Bandit Cole Younger | Edward L. Crain | 2:53 | |
| Ozark Bill | Jimmy Driftwood | 2:46 | |
| Sugar Hill | Mary Z. Cox | 3:33 | |
| Go Dig My Grave | Jean Ritchie and Doc Watson | 3:50 | |
| Sittin' On Top of the World | Sam Chatmon | 3:00 | |
| Don't Let Your Deal Go Down Blues | Charlie Poole with the North Carolina Ramblers | 2:53 | |
| A Spoonful Blues | Charley Patton | 3:12 | |
| Someday You'll Be Free | Frank Fairfield | 3:26 | |
| Twenty-One Years | The Lonesome Pine Fiddlers | 2:30 | |
| Sail Away Ladies | Uncle Dave Macon and His Fruit-Jar Drinkers | 3:00 | |
| Where is My Sailor Boy? (What Does the Deep Sea Say?) | Bill Monroe and Doc Watson | 3:31 | |
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| Jaybird March | Etta and Cora Baker | 1:50 | |
| Every Day Dirt | The Doc Watson Family | 2:08 | |
| Greasy Coat | Chance McCoy & The Appalachian String Band | 3:02 | |
| Cigarette Blues | Bo Carter | 3:18 | |
| Sail Away Lady - 2020 Remaster | Uncle Bunt Stephens | 2:58 | |
| Swanno Mountain | Roscoe Holcomb | 3:20 | |
| Wake Up Jacob - 2020 Remaster | Prince Albert Hunt's Texas Ramblers | 2:55 | |
| The Little Glass Of Wine | Stanley Brothers | 3:05 | |
| Cosmic Doo Doo | Blaze Foley | 1:48 | |
| Dry Bones - 2020 Remaster | Bascom Lamar Lunsford | 3:00 | |
| Bukka Jitterburg Swing | Bukka White | 2:35 | |
| The Devil's Dream | Blind Boy Paxton | 3:25 | |
| The Banks of the Arkansas / Wave the Ocean | Neil Morris | 1:58 | |
| Bright Sunny South | Dock Boggs | 3:41 | |
| Cocaine Done Kill My Baby | Mance Lipscomb | 2:33 | |
| You Get Down by the Pool Hall Clickety Clack - Sister Song | Michael Hurley | 3:34 | |
| Living The Right Life Now | Molly O'Day | 2:17 | |
| Last Payday at Coal Creek | Pete Steele | 3:03 | |
| Little Delia | Blind Willie McTell | 3:02 | |
| Willie Moore | The Kossoy Sisters | 3:05 | |
| Fishing Blues | Henry Thomas | 2:42 | |
| John Hardy | Frank Proffitt | 2:27 | |
| Rambling Hobo | Gaither Carlton | 1:43 | |
| Coal Tattoo | Hazel Dickens | 3:00 | |
| Kill It Kid - Bonus Track | Blind Willie McTell | 2:31 | |
| Cumberland Gap | Frank Proffitt | 1:25 | |
| Frankie Baker | Fred Cockerham | 1:22 | |
| Brilliancy Medley | Eck Robertson | 2:57 | |