| Shoo Turkey | Bessie Jones, Alan Lomax | 1:04 | |
| The Midnight Special | Ernest "Mexico" Williams, Alan Lomax | 1:45 | |
| That's No Way to Get Along (Alternate Take) | Robert Wilkins | 2:53 | |
| K.C. Moan | Memphis Jug Band | 2:32 | |
| Poor Man | Frank Proffitt | 3:02 | |
| It's Tight Like That | Tampa Red | 2:49 | |
| Huntington | Various Artists, Alan Lomax | 2:37 | |
| Aberdeen Mississippi Blues | Bukka White | 2:35 | |
| Johnnie O Braidislea | John Strachan, Alan Lomax | 1:32 | |
| Sink 'Em Low - Live | Georgia Sea Island Singers, Bessie Jones | 2:29 | |
| Going Across the Mountain | Frank Proffitt | 2:16 | |
| Wake Up Jacob - 2020 Remaster | Prince Albert Hunt's Texas Ramblers | 2:55 | |
| Cocaine (Original Mix) | Dick Justice | 3:01 | |
| Chilly Winds - Remastered | Wade Ward, Alan Lomax | 1:24 | |
| Special Streamline | Bukka White | 2:47 | |
| Oh Death | Dock Boggs | 3:22 | |
| Rabbit Chase | The New Lost City Ramblers | 2:36 | |
| Leather Britches | Sidney Carter, Alan Lomax | 1:48 | |
| Fishing Blues | Henry Thomas | 2:42 | |
| Eighteen Hammers | Johnny Lee Moore | 2:37 | |
| Cocaine Habit Blues | Memphis Jug Band | 2:48 | |
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| Newburgh | Various Artists, Alan Lomax | 1:58 | |
| Interview: Demonstration of Concert Guitar Tuning | Son House, Alan Lomax | 1:29 | |
| Dark Day - Remastered | Silver Leaf Quartet, Alan Lomax | 2:25 | |
| You're Gonna Need Somebody on Your Bond | Blind Willie Johnson | 3:09 | |
| I'm Going Home to Live With Jesus - Previously Unreleased | Viola James, Alan Lomax | 3:49 | |
| Black Woman | Alan Lomax | 2:58 | |
| Go Wash in the Beautiful Stream | Bessie Jones, Alan Lomax | 1:26 | |
| Caminito de la fuente (II) | Manuel Otero, Alan Lomax | 1:59 | |
| Church, I Know We Got Another Building | Ed Young, Lonnie Young Sr., G. D. Young, Alan Lomax | 2:20 | |
| A Spoonful Blues | Charley Patton | 3:12 | |
| Rainbow Mid Life's Willows - Remastered | Almeda Riddle, Alan Lomax | 2:51 | |
| Wise County Jail | Dock Boggs | 1:52 | |
| Tell It To Me | Grant Brothers & Their Music | 2:56 | |
| Every Day Dirt | The Doc Watson Family | 2:08 | |
| The Banks of the Arkansas / Wave the Ocean | Neil Morris | 1:58 | |
| Jesse James | Sid Hemphill, Alan Lomax | 1:41 | |
| Banging Breakdown - Remastered | Hobart Smith, Alan Lomax | 1:39 | |
| Screamin' and Hollerin' the Blues | Charley Patton | 3:05 | |
| Up Jumped The Rabbit | The Georgia Potlickers | 2:40 | |
| Goodbye Honey, You Call That Gone | Lucius Smith, Alan Lomax | 2:13 | |
| Morning Prayer | Various Artists, Alan Lomax | 2:33 | |
| Joseph Looney - Remastered | Ollie Gilbert, Alan Lomax | 2:25 | |
| White Lightinin' | Furry Lewis | 3:26 | |
| Old Blue | Furry Lewis | 3:00 | |
| I Am The Light Of This World | Rev. Gary Davis | 3:35 | |
| When You Go A-Curtin' | Alan Lomax | 2:13 | |
| Bagpipe Tunes Hail Ro Ruadh Waternish/Daldownie's Reel/The Spider In The Gaff/Slow Gaelic Air | John Burgess, Alan Lomax | 4:09 | |
| Interview #2 - Plantation Recording | Muddy Waters, Alan Lomax, John Work | 1:50 | |