| Guitar Rag | Sylvester Weaver | 3:06 | |
| Milwaukee Blues | Charlie Poole | 3:18 | |
| What's That Smells Like Fish | Blind Boy Fuller | 2:46 | |
| Walkin' Blues (Original Mix) | Son House | 2:52 | |
| Future Blues | Willie Brown | 3:01 | |
| The Whale Has Swallowed Me | J.B. Lenoir | 2:25 | |
| Mole in the Ground | Bascom Lamar Lunsford | 3:03 | |
| Sitting On Top Of The World | Mississippi Sheiks | 3:12 | |
| Bye Bye Baby Blues | Texas Alexander | 3:09 | |
| Black Cat Blues | Memphis Minnie | 2:54 | |
| Milk Cow Blues | Sleepy John Estes | 3:02 | |
| Nobody Knows You When Your Down and Out | Josh White | 3:00 | |
| God Don't Never Change | Blind Willie Johnson | 2:56 | |
| Seminole Blues | Tampa Red | 3:05 | |
| Yonder Comes The Blues | Stefan Grossman | 5:23 | |
| High Water Everywhere, Part 1 | Charley Patton | 2:59 | |
| Last Kind Words Blues (Alternate Take) | Geeshie Wiley | 3:02 | |
| Pin in Your Cushion | Bo Carter | 3:06 | |
| How Can a Poor Man Stand Such Times and Live | The New Lost City Ramblers | 3:35 | |
| When the Sun Goes Down | Leroy Carr | 2:59 | |
| Garter Snake Blues | Victoria Spivey | 3:17 | |
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| Good Biscuits | Memphis Minnie | 2:40 | |
| Henry Lee (Original Mix) | Dick Justice | 3:25 | |
| Lord I Just Can't Keep From Crying | Blind Willie Johnson | 3:00 | |
| Alabama March | J.B. Lenoir | 2:53 | |
| Country Blues | Dock Boggs | 3:55 | |
| Rag, Mama, Rag | Blind Boy Fuller | 3:03 | |
| All Around Man | Bo Carter | 3:00 | |
| Wake Up Jacob - 2020 Remaster | Prince Albert Hunt's Texas Ramblers | 2:55 | |
| Old Country Stomp | Henry Thomas | 2:51 | |
| Open Your Book, Your Daddy Got to Read With You | Sam Chatmon | 1:46 | |
| You Can't Get That Stuff No More | Tampa Red | 2:52 | |
| You Just as Well Let Her Go | Casey Bill Weldon, Kokomo Arnold | 3:09 | |
| County Jail Blues | Big Maceo | 2:55 | |
| Sixteen Tons | George Davis | 3:10 | |
| Going to Germany | Cannon's Jug Stompers | 2:31 | |
| Back Door Blues | Kokomo Arnold | 3:25 | |
| How Long, How Long Blues | Leroy Carr | 3:03 | |
| The Road Is Rough and Rocky | Archie Edwards | 3:27 | |
| Pure Religion | Rev. Gary Davis | 2:57 | |
| Devil Got My Woman | Skip James | 5:15 | |
| Little Boy Blue | Scrapper Blackwell | 3:07 | |
| I'm So Glad | Skip James | 1:56 | |
| Little Annie Mae | Big Joe Williams | 2:00 | |
| By The Spoonful | J.D. Short | 3:49 | |
| Washington D.C. Hospital Center Blues | Skip James | 4:11 | |
| White Lightinin' | Furry Lewis | 3:26 | |
| Graveyard Blues | Robert Pete Williams | 2:46 | |
| Bad Luck Blues | Blind Lemon Jefferson | 2:52 | |