| Mississippi Bottom Blues | Kid Bailey | 2:49 | |
| Black Snake Moan | Blind Lemon Jefferson | 2:50 | |
| I Know I Done You Wrong | Charles Caldwell | 2:49 | |
| 'Tain't Nobody's Business If I Do, Pt. 1 | Frank Stokes | 3:16 | |
| Diddie Wah Diddie | Blind Blake | 2:59 | |
| Parchman Farm Blues | Bukka White | 2:42 | |
| Walk On | Sonny Terry and Brownie McGhee | 3:11 | |
| Catfish Blues, No. 3 (Take 2) | Asie Payton | 3:48 | |
| Rollin' Stone, Pt. 1 | Robert Wilkins | 2:53 | |
| John Henry (The Steel Driving Man), Pt. 1 | Furry Lewis | 2:51 | |
| Yonder Comes The Blues | Stefan Grossman | 5:23 | |
| All of Your Love | Magic Sam, Earnest Johnson, Mighty Joe Young, Odie Payne, Jr., Stockholm Slim | 3:52 | |
| Aberdeen Mississippi Blues | Bukka White | 2:35 | |
| Terraplane Blues | Robert Johnson | 2:58 | |
| Old Buck | Charles Caldwell | 3:40 | |
| I Want Some of Your Pie | Blind Boy Fuller | 2:46 | |
| Bad Luck Blues (Alternative Take) | Lightnin' Slim | 2:35 | |
| Richmond Blues | Cephas & Wiggins, Phil Wiggins, John Cephas | 3:59 | |
| Stayed Awake | Robert Belfour | 3:39 | |
| Old Original Kokomo Blues | Kokomo Arnold | 2:54 | |
| Going to Germany | Cannon's Jug Stompers | 2:31 | |
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| I'm A Bad Luck Woman | Memphis Minnie | 3:00 | |
| Cross And Evil Woman Blues | Rev. Gary Davis | 3:02 | |
| Glory, Glory Hallelujah | Chester Davis and Congregation, Compton Jones and Group, Furry Lewis | 6:00 | |
| The Prodigal Son | Robert Wilkins | 10:00 | |
| Can't Hold Out Much Longer | Big Walter Horton | 2:52 | |
| It Hurts Me Too | Tampa Red | 2:33 | |
| Coal Black Mattie | Ranie Burnette | 4:43 | |
| If You See My Rooster (Please Run Him Home) | Memphis Minnie | 3:15 | |
| I Got My Eyes on You | Robert Belfour | 4:32 | |
| Keep It Clean | Charley Jordan | 2:48 | |
| Walking My Troubles Away | Blind Boy Fuller | 2:54 | |
| Fallin' Down Blues | Robert Wilkins | 2:38 | |
| Mean Ole Lonesome Train | Lightnin' Slim | 3:12 | |
| Blues For The Lowland | Sonny Terry and Brownie McGhee | 4:46 | |
| Bukka's Jitterbug Swing | Bukka White | 2:39 | |
| Cocaine Habit Blues | Memphis Jug Band | 2:48 | |
| Kissing in the Dark | Memphis Minnie | 2:31 | |
| You Don't Know My Mind | Pink Anderson | 2:39 | |
| Don't Let It Bother You | Leon Redbone | 2:58 | |
| Wonderin' And Worryin' | Slim Harpo | 2:08 | |
| Cornbread, Peas and Black Molasses | Sonny Terry and Brownie McGhee | 2:31 | |
| Denver Blues | Tampa Red | 2:54 | |
| Dig Myself a Hole | Arthur "Big Boy" Crudup | 2:23 | |
| Lester's Stomp | Lazy Lester | 1:53 | |
| Angola Penitentiary Blues | Robert Pete Williams | 7:07 | |
| Old Blue | Furry Lewis | 3:00 | |
| Little Annie Mae | Big Joe Williams | 2:00 | |
| Goin' To Kansas City | Furry Lewis | 2:53 | |