| Bring It With You When You Come | Cannon's Jug Stompers | 2:45 | |
| Kassie Jones, Pt. 1 | Furry Lewis | 3:07 | |
| Take Your Fingers off It | Will Shade, Charlie Burse, Gus Cannon | 2:54 | |
| Harlem Rag | Stefan Grossman | 1:29 | |
| Evil Devil Woman Blues | Memphis Minnie & Kansas Joe | 3:14 | |
| Cornbread, Peas and Black Molasses | Sonny Terry and Brownie McGhee | 2:31 | |
| The Butchers Boy - 2020 Remaster | Buell Kazee | 3:04 | |
| Sitting On Top Of The World | Mississippi Sheiks | 3:12 | |
| Take Your Burden to the Lord and Leave It There | Blind Willie Johnson | 2:55 | |
| Rope Stretching Blues | Blind Blake | 3:02 | |
| On the Road Again | Memphis Jug Band | 2:50 | |
| Shake It and Break It (But Don't Let It Fall Mama) | Charley Patton | 3:09 | |
| Cocaine Done Killed My Baby | Mance Lipscomb | 1:23 | |
| Denver Blues | Tampa Red | 2:54 | |
| Travelin' Higway Man | Tommy McClennan | 2:59 | |
| Special Streamline | Bukka White | 2:47 | |
| County Jail Blues | Big Maceo | 2:55 | |
| Revenue Man Blues | Charley Patton | 2:43 | |
| Catfish Blues | Corey Harris | 2:40 | |
| Moaning the Blues | Memphis Minnie | 3:06 | |
| Sugar Mama Blues | Sonny Boy Williamson I | 2:58 | |
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| When Can I Change My Clothes? | Bukka White | 2:59 | |
| The Half Ain't Never Been Told / Just Look / Come and See / God Moves on the Water / He Got Better Things for You / The Latter Rain Is Fall | Rev. F.W. McGee, Arizona Dranes, Rev. D.C. Rice, Blind Willie Johnson, Lonnie McIntorsh, Elder Edwards, The Memphis Sanctified Singers | 7:36 | |
| Rock Me Mama | Arthur "Big Boy" Crudup | 2:29 | |
| Evening Sun | Johnny Shines | 2:32 | |
| Oh Death | Charley Patton | 2:52 | |
| New Orleans (The Rising Sun Blues) | Lead Belly | 3:16 | |
| The Fox and the Hounds | Henry Thomas | 2:36 | |
| Ragged And Dirty | William Brown | 4:50 | |
| Take You Burden to The Lord and Leave It There | Blind Willie Johnson | 2:57 | |
| Drop Down Mama | Sleepy John Estes | 3:11 | |
| Going to Move to Alabama | Charley Patton | 3:03 | |
| Back Home Blues | Big Joe Williams | 4:22 | |
| Further Up on the Road | David "Honeyboy" Edwards | 4:20 | |
| I Am a Weary and a Lonesome Traveller | Barbara Dane and the Chambers Brothers | 4:17 | |
| I'm Gonna Run To The City Of Refuge | Blind Willie Johnson | 3:23 | |
| Cuba | Dock Boggs | 1:29 | |
| Hangman | Spider John Koerner, Dave Ray, Tony Glover | 2:32 | |
| Some Day Baby | Lonnie Johnson | 3:08 | |
| Cotton Crop Blues | James Cotton | 3:01 | |
| Mashing That Thing | Bo Carter | 2:40 | |
| Mississippi Heavy Water Blues | Roscoe Holcomb | 2:17 | |
| Dead Letter Blues | Son House | 3:31 | |
| It Hurts Me Too | Tampa Red | 2:33 | |
| She Left Me a Mule to Ride | Big Joe Williams | 2:35 | |
| The Banks of the Arkansas / Wave the Ocean | Neil Morris | 1:58 | |
| Skinny Leg Blues | Geeshie Wiley | 3:13 | |
| I Am The Light Of This World | Rev. Gary Davis | 3:35 | |
| Tryin' To Get Home | Rev. Gary Davis | 3:47 | |