| Graveyard Blues | Roscoe Holcomb | 3:01 | |
| Wake Up Jacob - 2020 Remaster | Prince Albert Hunt's Texas Ramblers | 2:55 | |
| Roll and Tumble Blues - 1929 Version | Hambone Willie Newbern | 3:04 | |
| Kassie Jones (Casey Jones) | Furry Lewis | 2:58 | |
| Ramblin' Blues | Charlie Poole | 3:07 | |
| Railroad Blues | Sam McGee | 2:48 | |
| Move on Down the Line | Jesse Fuller | 4:25 | |
| Cocaine Habit Blues | Memphis Jug Band | 2:48 | |
| John the Revelator | Blind Willie Johnson | 3:16 | |
| Satan, Your Kingdom Must Come Down | Frank Proffitt | 2:11 | |
| Ola Belle's Blues | Ola Belle Reed, Kevin Roth | 2:22 | |
| Big Road Blues | Tommy Johnson | 3:12 | |
| Last Payday at Coal Creek | Pete Steele | 3:03 | |
| Ground Hog | The Doc Watson Family | 2:21 | |
| Little Black Train | Dock Boggs | 2:55 | |
| Going Down the Road Feelin' Bad | Dom Flemons | 2:38 | |
| Walk Right In | Cannon's Jug Stompers | 2:55 | |
| High on a Mountain | Ola Belle Reed | 5:30 | |
| Cluck Old Hen | Frank Proffitt | 2:23 | |
| Take a Drink on Me | Charlie Poole | 3:17 | |
| Oh Death | Charley Patton | 2:52 | |
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| Lord, I Looked Down The Road | Leon Redbone | 3:04 | |
| I'm Gone Long Gone | Reno & Smiley | 2:25 | |
| Little Omie Wise | Dock Boggs | 3:26 | |
| Old Mountain Dew | The Delmore Brothers | 2:34 | |
| If I Had My Way | Rev. Gary Davis | 4:46 | |
| Cocaine Done Kill My Baby | Mance Lipscomb | 2:33 | |
| 'Tain't Nobody's Business If I Do, Pt. 1 | Frank Stokes | 3:16 | |
| I'll Fly Away | The Kossoy Sisters | 2:32 | |
| I Got to Cross the River Jordan | Blind Willie McTell | 4:38 | |
| I Get My Whiskey From Rockingham | Earl Johnson | 3:01 | |
| Ain't You Sorry | Mance Lipscomb | 2:20 | |
| I've Endured | Ola Belle Reed, Bud Reed, David Reed | 2:52 | |
| Pick Poor Robin Clean | Geeshie Wiley | 3:14 | |
| Down the Road | The Doc Watson Family | 1:41 | |
| Where is My Sailor Boy? (What Does the Deep Sea Say?) | Bill Monroe and Doc Watson | 3:31 | |
| Tallahatchie River Blues | Mattie Delaney | 2:48 | |
| Long Tall Girl Got Stuck on Me | Mance Lipscomb | 2:43 | |
| I'll Never Get Drunk No More | Frank Proffitt | 2:34 | |
| Big Railroad Blues | Cannon's Jug Stompers | 3:18 | |
| Tennessee Stud | Jimmie Driftwood | 3:21 | |
| If I Had My Way I'd Tear the Building Down | Blind Willie Johnson | 3:07 | |
| Daddy Walked in Darkness | Hoyt Axton | 2:49 | |
| Poor Ellen Smith | The Country Gentlemen | 2:16 | |
| Turn Your Radio On | The Blue Sky Boys, Bill Bolick, Earl Bolick | 1:49 | |
| Me and my uncle | Mike Wilhelm | 3:19 | |
| Cumberland Gap | Frank Proffitt | 1:25 | |
| A Thousand Miles From Nowhere | Robert Pete Williams | 5:14 | |
| Brilliancy Medley | Eck Robertson | 2:57 | |