| When The Levee Breaks | Memphis Minnie & Kansas Joe | 3:08 | |
| The Prodigal Son | Robert Wilkins | 10:00 | |
| Frankie & Johnny | Champion Jack Dupree | 3:23 | |
| The Butchers Boy - 2020 Remaster | Buell Kazee | 3:04 | |
| You Better Mind | Bessie Jones, Georgia Sea Island Singers, Alan Lomax | 2:14 | |
| The Lone Star Trail | Ken Maynard | 3:12 | |
| Riding In A Buggy | Vera Hall, Alan Lomax | 2:08 | |
| Bugger Burns | Spider John Koerner, Dave Ray, Tony Glover | 1:40 | |
| Stewball | Unidentified Mississippi State Penitentiary (Oakley) prisoners, Alan Lomax | 4:10 | |
| 'Tain't Nobody's Business If I Do, Pt. 1 | Frank Stokes | 3:16 | |
| Open Your Book, Your Daddy Got to Read With You | Sam Chatmon | 1:46 | |
| High Water Everywhere, Pt. 1 (Alternate Take) | Charley Patton | 3:04 | |
| Sugar Baby - 2020 Remaster | Dock Boggs | 2:58 | |
| John the Revelator - Alternate Take | Son House | 2:14 | |
| Father, I Stretch My Hands To Thee | Othar Turner | 2:01 | |
| Back Door Blues | Kokomo Arnold | 3:25 | |
| Sail Away Lady - 2020 Remaster | Uncle Bunt Stephens | 2:58 | |
| Talk to Your Daughter | J.B. Lenoir | 2:45 | |
| Rowdy Blues | Kid Bailey | 3:00 | |
| The World, She's Windin' Up | Cora Fluker, Ola Mae Bell | 2:51 | |
| I Know His Blood Can Make Me Whole | Blind Willie Johnson | 3:02 | |
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| You Got To Walk That Lonesome Valley | Rev. F.W. McGee | 3:32 | |
| Red River Blues | Henry Thomas | 3:04 | |
| Rope Stretching Blues | Blind Blake | 3:02 | |
| Railroad Bill - Remastered | Hobart Smith, Alan Lomax | 2:42 | |
| Trying to Make It Home | Robert Johnson | 2:57 | |
| Moses Don't Get Lost | Bessie Jones, Alan Lomax | 2:24 | |
| Feelin' Good | J.B. Lenoir | 3:01 | |
| Pearline | Son House | 4:31 | |
| Going to Germany | Cannon's Jug Stompers | 2:31 | |
| I Be so Glad When the Sun Goes Down | Ed Lewis | 2:36 | |
| Church, I Know We Got Another Building | Ed Young, Lonnie Young Sr., G. D. Young, Alan Lomax | 2:20 | |
| Lonesome Blues | Alan Lomax | 3:29 | |
| Move This Rope | J.B. Lenoir | 3:34 | |
| Snake Baked a Hoecake | Peggy Seeger | 1:06 | |
| You Got to Die | Blind Willie McTell | 3:12 | |
| Jesus Make Up My Dying Bed | Blind Willie Johnson | 3:10 | |
| Born Dead | J.B. Lenoir | 3:47 | |
| Chevrolet | Ed Young, Lonnie Young, G.D. Young | 4:07 | |
| The 12 Year Old Boy | Elmore James | 3:06 | |
| Preachin' Blues (Up Jumped the Devil) | Robert Johnson | 2:48 | |
| Mean Old Frisco Blues - Remastered 2003 | Arthur "Big Boy" Crudup | 2:35 | |
| Eisenhower Blues | J.B. Lenoir | 2:52 | |
| Chevrolet | Lonnie Young | 2:52 | |
| Black Cat Bone | Jessie Mae Hemphill | 4:57 | |
| No Place To Go (You Gonna Wreck My Life) | Howlin' Wolf | 2:58 | |
| Standing In My Doorway Crying | Jessie Mae Hemphill | 4:40 | |
| Rollin' And Tumblin' | Big Joe Williams | 2:36 | |
| A Thousand Miles From Nowhere | Robert Pete Williams | 5:14 | |
