| Jim and John | Ed Young, Lonnie Young, G.D. Young | 3:08 | |
| I was Healed by the Wounds in His Side | Sister Gertrude Morgan | 1:34 | |
| I'm Going Home - Remastered | Ervin Webb, Unidentified prisoners, Alan Lomax | 3:25 | |
| I'm A Steady Rollin' Man | Robert Johnson | 2:38 | |
| Going Down Slow | St. Louis Jimmy Oden | 3:11 | |
| Dead Letter Blues | Son House | 3:31 | |
| Baby You Gotta Change Your Mind | Blind Boy Fuller | 3:12 | |
| Bye and Bye I'm Goin' to See the King | Blind Willie Johnson | 2:51 | |
| Early In the Morning - Remastered | Unidentified prisoners, Johnny Lee Moore, Alan Lomax | 2:54 | |
| Snake Baked a Hoecake | Peggy Seeger | 1:06 | |
| Stewball | Unidentified Mississippi State Penitentiary (Oakley) prisoners, Alan Lomax | 4:10 | |
| Walk Right In | Cannon's Jug Stompers | 2:55 | |
| I'll Be Rested (When The Roll Is Called) | Blind Roosevelt Graves | 2:33 | |
| Blue Ghost Blues | Lonnie Johnson | 3:09 | |
| Downtown Blues | Frank Stokes | 3:17 | |
| Drunkard's Lone Child | Dock Boggs | 4:10 | |
| Southbound | The Doc Watson Family | 2:40 | |
| Sagefield Woman Blues | Kokomo Arnold | 3:04 | |
| Keep My Skillet Good and Greasy | Uncle Dave Macon | 2:46 | |
| Preachin' Blues (Up Jumped the Devil) | Robert Johnson | 2:48 | |
| Sugar Diabetes Blues | Big Joe Williams | 2:46 | |
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| That's All Right, Pt. 1 | John Henry Barbee | 3:40 | |
| The Coo-Coo Bird | Doc Watson | 2:36 | |
| Work With Me Annie | Hank Ballard | 3:05 | |
| Buzzard Lope | Bessie Jones, Georgia Sea Island Singers, Alan Lomax | 1:59 | |
| Mashing That Thing | Bo Carter | 2:40 | |
| Walking Blues | Son House, Willie Brown, Fiddlin’ Joe Martin, Leroy Williams | 6:35 | |
| I Am a Weary and a Lonesome Traveller | Barbara Dane and the Chambers Brothers | 4:17 | |
| I Want Jesus to Walk with Me | James Shorty | 3:43 | |
| Get Your Yas Yas Out | Blind Boy Fuller | 2:28 | |
| Sundown | Son House | 6:12 | |
| Baby Please Don't Go | Big Joe Williams | 2:44 | |
| None But the Righteous - Remastered | Union Choir of the Church of God and Saints of Christ, Alan Lomax | 3:07 | |
| Cigarette Blues | Bo Carter | 3:18 | |
| Duckin' and Dodgin' | Alan Lomax | 3:37 | |
| Ham & Eggs | Danny Barker | 2:22 | |
| Bukka's Jitterbug Swing | Corey Harris | 2:00 | |
| Sittin' On Top of the World | Sam Chatmon | 3:00 | |
| Back Home Blues | Big Joe Williams | 4:22 | |
| Evening Sun | Johnny Shines | 2:32 | |
| Praise God I'm Satisfied | Blind Willie Johnson | 3:10 | |
| Laughing to Keep from Crying | Mississippi Joe Callicott | 3:44 | |
| Old Dog Blue | Jim Jackson | 2:58 | |
| Dead Drunk Blues | Ma Rainey | 2:52 | |
| I Am The Light Of This World | Rev. Gary Davis | 3:35 | |
| Little Boy Blue | Scrapper Blackwell | 3:07 | |
| Great Change Since I Been Born | Rev. Gary Davis | 4:03 | |
| Here In The Dark | T-Bone Walker | 3:01 | |
| Graveyard Blues | Robert Pete Williams | 2:46 | |
