| Rope Stretching Blues | Blind Blake | 3:02 | |
| Memphis Rounders Blues | Frank Stokes | 2:51 | |
| Billy Lyons and Stack O'lee | Furry Lewis | 2:31 | |
| Jesus On the Mainline - Remastered | James Shorter, Viola James | 3:40 | |
| Tribulations - Remastered | E.C. Ball | 2:50 | |
| Up Jumped The Rabbit | The Georgia Potlickers | 2:40 | |
| I Crave My Pig Meat | Blind Boy Fuller | 2:51 | |
| Cottonfield Blues, Pt. 2 | Garfield Akers | 3:21 | |
| Someday Baby Blues | Sleepy John Estes | 2:59 | |
| Praise God I'm Satisfied | Blind Willie Johnson | 3:10 | |
| The 12 Year Old Boy | Elmore James | 3:05 | |
| I Could Cry (1957 Version) | Junior Wells | 3:10 | |
| The Banks of the Arkansas / Wave the Ocean | Neil Morris | 1:58 | |
| The Butchers Boy - 2020 Remaster | Buell Kazee | 3:04 | |
| I'm Going Home - Remastered | Ervin Webb, Unidentified prisoners | 3:25 | |
| Mashing That Thing | Bo Carter | 2:40 | |
| Sail Away Lady - 2020 Remaster | Uncle Bunt Stephens | 2:58 | |
| Dry Bones - 2020 Remaster | Bascom Lamar Lunsford | 3:00 | |
| Floating Bridge | Sleepy John Estes | 3:12 | |
| Keep It Clean | Charley Jordan | 2:48 | |
| Rock Me Mama | Alan Lomax | 3:34 | |
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| I Got Mine | Frank Stokes | 3:06 | |
| West Coast Blues | Blind Blake | 3:08 | |
| New Jerusalem | Sister Gertrude Morgan | 2:23 | |
| Milk Cow Blues | Sleepy John Estes | 3:02 | |
| That's All Right, Pt. 1 | John Henry Barbee | 3:40 | |
| Jumpin' Judy | Alan Lomax | 4:11 | |
| The Prodigal Son | Robert Wilkins | 10:00 | |
| Mean Ol' Frisco | Arthur "Big Boy" Crudup | 2:37 | |
| Move This Rope | J.B. Lenoir | 3:34 | |
| Banana in Your Fruit Basket | Bo Carter | 3:08 | |
| Lord I Just Can't Keep From Crying | Blind Willie Johnson | 3:00 | |
| Downtown Blues | Frank Stokes | 3:17 | |
| Tell It To Me | Grant Brothers & Their Music | 2:56 | |
| Big Railroad Blues | Cannon's Jug Stompers | 3:18 | |
| Never No Mo' Blues | Jimmie Rodgers | 2:41 | |
| Tangle Eye Blues | Alan Lomax | 3:06 | |
| Last Dime Blues | Blind Willie McTell | 2:49 | |
| Linin' Track | Spider John Koerner, Dave Ray, Tony Glover | 2:19 | |
| God's Word Will Never Pass Away | Sister Gertrude Morgan | 1:22 | |
| Back Door Blues | Kokomo Arnold | 3:25 | |
| Alabama Jubilee | R. Crumb And His Cheap Suit Serenaders | 2:40 | |
| Walking Blues | Son House, Willie Brown, Fiddlin’ Joe Martin, Leroy Williams | 6:35 | |
| Jim and John | Lonnie Young | 3:09 | |
| By The Spoonful | J.D. Short | 3:49 | |
| Hen Duck | Lonnie Young | 3:07 | |
| Another Man Done Gone | Vera Hall | 1:24 | |
| The Cat's Dreamin' | Clifton Chenier | 2:08 | |
| Old Blue | Furry Lewis | 3:00 | |