| Sweet Violets | Harry McClintock | 1:22 | |
| The Wild Rippling Water | Alan Lomax | 3:11 | |
| I'll Be Rested (When The Roll Is Called) | Blind Roosevelt Graves | 2:33 | |
| Gabriel's Call | Hazel Dickens, Alice Gerrard | 2:16 | |
| Johnny Booker | The New Lost City Ramblers, Cousin Emmy | 1:32 | |
| Bowling Green | The Kossoy Sisters | 2:13 | |
| Don’t You See That Train | The Delmore Brothers | 3:12 | |
| Rocky Road To Dublin | Chance McCoy & The Appalachian String Band | 1:35 | |
| Spike Driver Blues | Mississippi John Hurt | 3:13 | |
| Napoleon Crossing The Rhine | Mary Z. Cox | 1:19 | |
| Sugar Baby | Dock Boggs | 2:57 | |
| Moonlight Waltz | Tiny Moore, Jethro Burns | 3:34 | |
| Twinkle, Twinkle, Little Star | Snuffy Jenkins, Homer "Pappy" Sherrill | 1:18 | |
| John Hardy | Frank Proffitt | 2:27 | |
| Banks O' Doon | Robin Roberts | 2:02 | |
| Abe's Retreat | John Blisard | 2:01 | |
| 1928 | Charlie Parr | 4:08 | |
| The Little Glass Of Wine | Stanley Brothers | 3:05 | |
| Dark Holler Blues | Clarence Ashley | 2:58 | |
| Fishing Blues | Henry Thomas | 2:42 | |
| Wild Bill Jones | Frank Proffitt | 3:27 | |
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| jd plays clyde davenport plays an unnamed dee hicks tune | Joseph Decosimo | 1:23 | |
| The Golden Vanity | Jody Stecher | 5:28 | |
| Waynesboro | Chance McCoy & The Appalachian String Band | 3:33 | |
| Bluegrass Banjo | Erik Darling, The Kossoy Sisters, Robin Roberts | 1:41 | |
| Omie Wise | Doug and Jack Wallin | 3:05 | |
| Down South Blues | Dock Boggs | 2:11 | |
| Nine Pound Hammer | Clarence White | 1:24 | |
| Go Dig My Grave | Jean Ritchie and Doc Watson | 3:50 | |
| The Banks of the Arkansas / Wave the Ocean | Neil Morris | 1:58 | |
| Reuben Train | Frank Proffitt | 2:57 | |
| Coal Miner's Blues - 2021 Remaster | Hazel Dickens, Alice Gerrard | 2:42 | |
| Rock About Saro Jane | Uncle Dave Macon | 3:23 | |
| Little Maggie | Barbara Dane | 2:09 | |
| Hard and It's Hard | Robin Roberts | 2:16 | |
| What Will We Do with the Baby O | The Kossoy Sisters | 1:46 | |
| Long Haired Preacher (Preacher and the Slave) | Harry McClintock | 3:18 | |
| Down On Penny's Farm | Bentley Boys | 2:48 | |
| Nora | Nathan Sefalden | 3:09 | |
| Cuba | Dock Boggs | 1:29 | |
| Won't You Come and Sing for Me? - 2021 Remaster | Hazel Dickens, Alice Gerrard | 2:45 | |
| Way Down The Old Plank Road | Uncle Dave Macon, Sam McGee | 2:58 | |
| Reno Ride | Clarence White | 1:05 | |
| Suwannee River | Mary Z. Cox | 0:57 | |
| Dance All Night With A Bottle In Your Hand | Chance McCoy & The Appalachian String Band | 2:56 | |
| Charmin' Betsy | Henry Thomas | 3:01 | |
| Dog Blue - Instrumental | Mimi And Richard Farina | 1:46 | |
| Empty Pocket Blues | Hot Rize | 2:20 | |
| Soldier's Joy | The Kentucky Colonels | 1:14 | |