| East Tennessee Blues | Bill Monroe and Doc Watson | 2:12 | |
| Mole in the Ground | Bascom Lamar Lunsford | 3:03 | |
| Sweet Sweet Thing | The Delmore Brothers | 2:52 | |
| Call Me A Dog When I'm Gone | Frank Fairfield | 2:24 | |
| Sugar Babe | Mance Lipscomb | 2:06 | |
| Little Maggie | Barbara Dane | 2:09 | |
| Green Corn | Billy Faber | 1:41 | |
| The Devil's Dream | Blind Boy Paxton | 3:25 | |
| New Prisoner's Song | Dock Boggs | 2:57 | |
| Watson's Blues | Bill Monroe and Doc Watson | 2:28 | |
| Cocaine Done Kill My Baby | Mance Lipscomb | 2:33 | |
| True Love | Roscoe Holcomb | 6:23 | |
| Nine Pound Hammer | Jim & Jesse | 2:05 | |
| Omie Wise | Doug and Jack Wallin | 3:05 | |
| Railroad Blues | Sam McGee | 2:48 | |
| Shelvin Rock | Chance McCoy & The Appalachian String Band | 3:08 | |
| Minglewood Blues | Cannon's Jug Stompers | 3:40 | |
| Fishing Blues | Henry Thomas | 2:42 | |
| Billy Lyons and Stack O'lee | Furry Lewis | 2:31 | |
| Baby Let Your Hair Roll Down | Roscoe Holcomb | 2:33 | |
| Took My Gal a-Walkin' | Charlie Poole | 2:48 | |
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| Little Maggie | Iron Mountain String Band | 3:10 | |
| Hesitation | Reverend Gary Davis | 3:18 | |
| T.B. Blues (They're at Rest Together) | Hazel Dickens, Alice Gerrard | 3:28 | |
| Kings County Breakdown | Frank Fairfield | 3:04 | |
| The Butchers Boy - 2020 Remaster | Buell Kazee | 3:04 | |
| Billy in the Low Ground | Clarence White | 1:37 | |
| Moonshiner | Roscoe Holcomb | 2:00 | |
| A Home in Old Kentucky | Cousin Emmy, The New Lost City Ramblers | 1:30 | |
| Boil Them Cabage Down | Curly King & The Tennessee Hilltoppers | 1:04 | |
| Ommie Wise - 2020 Remaster | G. B. Grayson | 3:13 | |
| What Will We Do with the Baby O | The Kossoy Sisters | 1:46 | |
| John Brown's March (feat. Patti Walker) | Mike Oberst | 2:58 | |
| Ragged But Right | Riley Puckett | 2:57 | |
| 'Tain't Nobody's Business If I Do, Pt. 1 | Frank Stokes | 3:16 | |
| Church, I'm Fully Saved Today | Blind Willie Johnson | 3:06 | |
| Out on the Open West | Frank Fairfield | 3:13 | |
| Hungry Hash House | Charlie Poole | 3:25 | |
| Rocky Road To Dublin | Chance McCoy & The Appalachian String Band | 1:35 | |
| Fair Miss in the Garden | Roscoe Holcomb | 3:53 | |
| Cotton Eyed Joe | The New Lost City Ramblers | 1:15 | |
| Peg and Awl | Carolina Tar Heels | 2:57 | |
| Captain Kidd | Michael Hurley | 2:16 | |
| Married Life Blues | Roscoe Holcomb | 1:51 | |
| Sugar Hill | Mary Z. Cox | 3:33 | |
| My Old Horse Died | Dock Boggs | 1:48 | |
| Tryin' To Get Home | Rev. Gary Davis | 3:47 | |
| Long Journey Home | Don Reno, Bill Harrell, The Tennessee Cut-Ups | 2:00 | |
| A Beautiful Life | The Kentucky Colonels | 1:59 | |