| Every Day Dirt | The Doc Watson Family | 2:08 | |
| Intersoular Blues | Michael Hurley | 4:05 | |
| Captain, Captain - Version; Take 2; Remastered | Mance Lipscomb | 2:34 | |
| Little Laura Blues | Sleepy John Estes | 2:31 | |
| Up Jumped The Rabbit | The Georgia Potlickers | 2:40 | |
| High Sheriff Blues | Charley Patton | 3:10 | |
| If I Could Be the Rain | Rosalie Sorrels | 2:55 | |
| K.C. Moan | Memphis Jug Band | 2:32 | |
| Take My Hand, Lead Me On | Sister Gertrude Morgan | 2:36 | |
| Bottleneck Blues | Sylvester Weaver | 2:56 | |
| My Old Horse Died | Dock Boggs | 1:48 | |
| Take Your Burden to the Lord and Leave It There | Washington Phillips | 3:15 | |
| High Water Everywhere, Pt. 1 (Alternate Take) | Charley Patton | 3:04 | |
| Aberdeen Mississippi Blues | Bukka White | 2:35 | |
| You Got to Die | Blind Willie McTell | 3:12 | |
| 'Tain't Nobody's Business If I Do, Pt. 1 | Frank Stokes | 3:16 | |
| Bring It With You When You Come | Cannon's Jug Stompers | 2:45 | |
| Oh Death | Charley Patton | 2:52 | |
| Tangle Eye Blues | Alan Lomax | 3:06 | |
| Oh Glory, How Happy I Am | Rev. Gary Davis | 4:36 | |
| Swannanoa Tunnel | Bascom Lamar Lunsford | 3:00 | |
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| Girl of Constant Sorrow | Barbara Dane | 4:06 | |
| The World, She's Windin' Up | Cora Fluker, Ola Mae Bell | 2:51 | |
| Black Woman | Alan Lomax | 2:58 | |
| Don't Ease Me In | Henry Thomas | 2:58 | |
| Good Morning Judge | Furry Lewis | 5:31 | |
| Ain't It Grand to Live a Christian Life | Blind Willie McTell | 2:38 | |
| Stop Look & Listen | Shirley Ann Lee | 2:52 | |
| High Water Everywhere, Pt. 1 | Charley Patton | 3:08 | |
| The Coo-Coo Bird | Doc Watson | 2:36 | |
| You Missed a Good Man | Tampa Red | 3:34 | |
| Prayer Song | Vera Hall, Dock Reed | 2:37 | |
| Rag, Mama, Rag | Blind Boy Fuller | 3:03 | |
| Minglewood Blues | Cannon's Jug Stompers | 3:40 | |
| Long Lonesome Road | Peggy Seeger | 2:17 | |
| Open Your Book, Your Daddy Got to Read With You | Sam Chatmon | 1:46 | |
| When Can I Change My Clothes? | Bukka White | 2:59 | |
| Whoopee Blues | King Solomon Hill | 3:11 | |
| Down South Blues | Dock Boggs | 2:11 | |
| Riding In A Buggy - Previously Unreleased | Vera Hall | 2:08 | |
| Prettiest Train | Alan Lomax | 3:43 | |
| Tree Blues | Olu Dara | 2:34 | |
| When the Train Comes Along | Henry Thomas | 2:56 | |
| Country Blues (No. 1) - Plantation Recording | Muddy Waters | 3:33 | |
| So Glad I'm Here | Bessie Jones | 1:46 | |
| She-Wolf | Jessie Mae Hemphill | 4:41 | |
| I Been Drinking | Vera Hall | 2:44 | |
| Another Man Done Gone - Onetram Remix | Adam Fearn, Vera Hall, Onetram | 6:20 | |
| Reconciled To The Blues | Michael Hurley | 3:02 | |