| Cigarette Blues | Bo Carter | 3:18 | ||
| Drop Down Mama | Sleepy John Estes | 3:11 | ||
| Down South Blues | Dock Boggs | 2:11 | ||
| Rope Stretching Blues | Blind Blake | 3:02 | ||
| Take Me Back | Corey Harris | 2:19 | ||
| Jesus Make Up My Dying Bed | Blind Willie Johnson | 3:10 | ||
| Prayer of Death, Pt. 1 | Charley Patton | 3:06 | ||
| Mississippi Heavy Water Blues | Roscoe Holcomb | 2:17 | ||
| Another Man Done Gone | Vera Hall | 1:27 | ||
| Mistreated Mama Blues | Dock Boggs | 1:54 | ||
| You Were Sent to This World | Johnnie Frierson | 3:09 | ||
| Aberdeen Mississippi Blues | Bukka White | 2:35 | ||
| Let Me Tell You | Marvin Pontiac | 2:38 | ||
| Wake Up Jacob - 2020 Remaster | Prince Albert Hunt's Texas Ramblers | 2:55 | ||
| Swing Low Sweet Chariot | Sister O.M. Terrell | 2:36 | ||
| Jane Jane | Peggy Seeger | 1:32 | ||
| Go Ahead, Buddy | Casey Bill Weldon, Kokomo Arnold | 2:44 | ||
| Travelin' Blues | Blind Willie McTell | 3:14 | ||
| Sometimes | Bessie Jones | 0:54 | ||
| Take Your Burden To The Lord And Leave It There | Washington Phillips | 3:15 | ||
| Ain't You Sorry | Mance Lipscomb | 2:20 | ||
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| Summertime | Lonnie Johnson | 2:13 | ||
| Garter Snake Blues | Victoria Spivey | 3:17 | ||
| High Water Everywhere, Pt. 1 (Alternate Take) | Charley Patton | 3:04 | ||
| John Henry | Henry Thomas | 2:43 | ||
| Greenback Dollar | Weems String Band | 3:10 | ||
| All I Have To Depend On | Shirley Ann Lee | 3:54 | ||
| Church, I'm Fully Saved Today | Blind Willie Johnson | 3:06 | ||
| Murder's Home | Alan Lomax | 0:50 | ||
| Henry Lee (Original Mix) | Dick Justice | 3:25 | ||
| Down in Mississippi | J.B. Lenoir | 3:16 | ||
| 'Tain't Nobody's Business If I Do, Pt. 1 | Frank Stokes | 3:16 | ||
| I'm A Bad Luck Woman | Memphis Minnie | 3:00 | ||
| Little Laura Blues | Sleepy John Estes | 2:31 | ||
| Graveyard Blues | Roscoe Holcomb | 3:01 | ||
| Blow, Gabriel | The McIntosh County Shouters | 3:37 | ||
| How Long | Frank Stokes | 3:18 | ||
| Kissing in the Dark | Memphis Minnie | 2:31 | ||
| Boll Weevil | Vera Hall | 1:14 | ||
| Last Kind Words Blues (Alternate Take) | Geeshie Wiley | 3:02 | ||
| Diamond Joe | Bessie Jones, Alan Lomax | 1:19 | ||
| Alabama March | J.B. Lenoir | 2:53 | ||
| Carrie | Vera Hall | 3:37 | ||
| Haunted House | Lonnie Johnson, Elmer Snowden | 5:00 | ||
| Pure Religion | Rev. Gary Davis | 2:57 | ||
| Greasy Greens | Pink Anderson | 4:38 | ||
| My Epitaph | Ola Belle Reed | 3:10 | ||
| John Henry | Furry Lewis | 5:49 | ||
| Job, Job | Dock Reed, Vera Hall | 2:46 | ||
