| Minglewood Blues | Cannon's Jug Stompers | 3:40 | |
| Windham | Alabama Sacred Harp Singers | 2:00 | |
| Black Woman | Alan Lomax | 2:58 | |
| Snake Baked a Hoecake | Peggy Seeger | 1:06 | |
| Graveyard Blues | Roscoe Holcomb | 3:01 | |
| Black Woman | Vera Hall, D. W. Bama Stuart | 4:46 | |
| Down in Mississippi | J.B. Lenoir | 3:16 | |
| She's A Bread Baker | C.W. Stoneking | 3:17 | |
| I'll Be Rested (When The Roll Is Called) | Blind Roosevelt Graves | 2:33 | |
| So Different Blues | Mance Lipscomb | 3:05 | |
| Nobody's Fault But Mine | Johnny Shines | 4:45 | |
| My Old Horse Died | Dock Boggs | 1:48 | |
| High Water Everywhere, Pt. 1 (Alternate Take) | Charley Patton | 3:04 | |
| Strange Place Blues | Bukka White | 2:52 | |
| Henry Lee (Original Mix) | Dick Justice | 3:25 | |
| Boll Weevil Holler | Vera Hall | 2:03 | |
| House Carpenter | Peggy Seeger | 4:08 | |
| Born Dead | J.B. Lenoir | 3:47 | |
| Prettiest Train | Alan Lomax | 3:43 | |
| Candy Gal | Vera Hall, Martha Owens, Cecil Owens | 3:10 | |
| Rocky Road | Alabama Sacred Harp Singers | 2:46 | |
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| I Just Want To Make Love To You | Barbara Dane | 2:14 | |
| Diamond Joe | Bessie Jones, Alan Lomax | 1:19 | |
| Down South Blues | Dock Boggs | 2:11 | |
| Judge Harsh Blues | Furry Lewis | 3:05 | |
| John the Revelator | Son House | 2:29 | |
| Baltimore Oriole | Judy Henske | 2:24 | |
| Keep Your Lamp Trimmed And Burning | Blind Willie Johnson | 3:02 | |
| Another Man Done Gone | Vera Hall | 1:27 | |
| Intersoular Blues | Michael Hurley | 4:05 | |
| Wake Up Jacob - 2020 Remaster | Prince Albert Hunt's Texas Ramblers | 2:55 | |
| 'Tain't Nobody's Business If I Do, Pt. 1 | Frank Stokes | 3:16 | |
| Linin' Track | Spider John Koerner, Dave Ray, Tony Glover | 2:19 | |
| I Wish I Was a Mole in the Ground | Bascom Lamar Lunsford | 3:22 | |
| Boll Weevil | Vera Hall | 1:14 | |
| On That Rock | Viola James | 2:56 | |
| Buck Dance - Version; Instrumental; Remastered | Mance Lipscomb | 1:33 | |
| Death | Vera Hall, Frances Anderson | 3:27 | |
| Take Your Burden to the Lord and Leave It There | Washington Phillips | 3:15 | |
| Whoopee Blues | King Solomon Hill | 3:11 | |
| Old Country Stomp | Henry Thomas | 2:51 | |
| I've Grown So Ugly | Robert Pete Williams | 2:47 | |
| So Glad I'm Here | Bessie Jones | 1:46 | |
| Black Cat Bone | Jessie Mae Hemphill | 4:57 | |
| Straight Street | The Pilgrim Travelers | 2:17 | |
| Be My Woman - Take 1 | Terry Callier | 5:45 | |
| Empire State Express | Son House | 4:30 | |
| Greasy Greens | Pink Anderson | 4:38 | |
| Another Man Done Gone - Onetram Remix | Adam Fearn, Vera Hall, Onetram | 6:20 | |