| Shake It and Break It (But Don't Let It Fall Mama) | Charley Patton | 3:09 | |
| Cool Drink Of Water | Houston Stackhouse | 3:16 | |
| Kassie Jones (Casey Jones) | Furry Lewis | 2:58 | |
| Railroad Blues | Sam McGee | 2:48 | |
| Drop Down Mama | Sleepy John Estes | 3:11 | |
| Indian War Whoop | Hoyt Ming and His Pep Steppers | 3:11 | |
| Milwaukee Blues | Charlie Poole | 3:18 | |
| Pony Blues | Charley Patton | 2:59 | |
| Georgia Bound | Blind Blake | 3:22 | |
| On The Road Again | Memphis Jug Band | 2:48 | |
| Dry Bones - 2020 Remaster | Bascom Lamar Lunsford | 3:00 | |
| Coal Miner's Blues - 2021 Remaster | Hazel Dickens, Alice Gerrard | 2:42 | |
| Georgia Buck | Precious Bryant | 2:38 | |
| Ground Hog | The Doc Watson Family | 2:21 | |
| I'll Go with Her | Robert Wilkins | 3:06 | |
| Black Snake Moan | Blind Lemon Jefferson | 2:50 | |
| The Butchers Boy - 2020 Remaster | Buell Kazee | 3:04 | |
| Further Up on the Road | David "Honeyboy" Edwards | 4:20 | |
| Guitar Rag | Sylvester Weaver | 3:06 | |
| The Road Is Rough and Rocky | Archie Edwards | 3:27 | |
| Walk Right In | Cannon's Jug Stompers | 2:55 | |
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| Wake Up Jacob - 2020 Remaster | Prince Albert Hunt's Texas Ramblers | 2:55 | |
| I Got to Cross the River Jordan | Blind Willie McTell | 4:38 | |
| Poor Man | Frank Proffitt | 3:02 | |
| Sitting On Top Of The World | Mississippi Sheiks | 3:12 | |
| What Happens In Bristol (Spoken Word) | Neil Morris | 2:25 | |
| Devil Sent the Rain Blues | Charley Patton | 3:05 | |
| Diddie Wa Diddie | Blind Blake | 3:00 | |
| On That Rock | Viola James | 2:56 | |
| Sugar Baby | Dock Boggs | 2:57 | |
| She's A Bread Baker | C.W. Stoneking | 3:17 | |
| Ain't It Grand to Live a Christian Life | Blind Willie McTell | 2:38 | |
| St. James Infirmary | Josh White | 3:42 | |
| Far Away | Neil Morris | 3:48 | |
| Banjo Tune | Erik Darling, The Kossoy Sisters, Robin Roberts | 1:42 | |
| Beaver Dam Road | Frank Proffitt | 1:26 | |
| The Twelves (The Dirty Dozens) | Casey Bill Weldon, Kokomo Arnold | 3:11 | |
| Storms are on the Ocean | Jean Ritchie and Doc Watson | 3:26 | |
| Lucky Holler | Ed Lewis | 2:20 | |
| Church Bells Blues | Luke Jordan | 3:15 | |
| When The Saints Go Marchin' In | Othar Turner, The Rising Star Fife, Drum Band | 3:17 | |
| Snake Baked a Hoecake | Peggy Seeger | 1:06 | |
| A Spoonful Blues | Charley Patton | 3:12 | |
| Harlem Rag | Stefan Grossman | 1:29 | |
| Crawdad Hole | Jessie Mae Hemphill | 2:07 | |
| Cumberland Gap | Frank Proffitt | 1:25 | |
| Pure Religion | Rev. Gary Davis | 2:57 | |
| John Henry | Furry Lewis | 5:49 | |
| Wednesday Night Waltz | Clark Kessinger | 3:08 | |