| Tallahatchie River Blues | Mattie Delaney | 2:48 | |
| Red River Blues | Henry Thomas | 3:04 | |
| Nobody Knows When You're Down | Big Joe Williams | 3:26 | |
| Kissing in the Dark | Memphis Minnie | 2:31 | |
| Screamin' and Hollerin' the Blues | Charley Patton | 3:05 | |
| Down in Mississippi | J.B. Lenoir | 3:16 | |
| Lovesick Blues | Emmett Miller | 2:53 | |
| Pick Poor Robin Clean | Geeshie Wiley | 3:14 | |
| Cornbread, Peas and Black Molasses | Sonny Terry and Brownie McGhee | 2:31 | |
| Say No to the Devil | Rev. Gary Davis | 3:20 | |
| John the Revelator | Son House | 2:29 | |
| Running Wild Blues | Charley Patton | 2:53 | |
| Cocaine Habit Blues | Memphis Jug Band | 2:48 | |
| Best Jockey in Town | Lonnie Johnson | 2:57 | |
| Travelin' Higway Man | Tommy McClennan | 2:59 | |
| County Jail Blues | Big Maceo | 2:55 | |
| County Farm Blues | Son House | 2:12 | |
| Lord I'm Discouraged | Charley Patton | 3:08 | |
| Stool Pigeon Blues | Big Joe Williams | 3:14 | |
| Garter Snake Blues | Victoria Spivey | 3:17 | |
| Trouble Mind | Sonny Terry and Brownie McGhee | 3:26 | |
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| High Water Everywhere, Pt. 2 (Alternate Take) | Charley Patton | 3:04 | |
| Walking Blues | Son House, Willie Brown, Fiddlin’ Joe Martin, Leroy Williams | 6:35 | |
| Floating Bridge | Sleepy John Estes | 3:12 | |
| Tears Don't Fall No More | Lonnie Johnson | 3:11 | |
| Big Railroad Blues | Cannon's Jug Stompers | 3:18 | |
| Nobody's Fault But Mine | Johnny Shines | 4:45 | |
| Fixin' to Die Blues | Bukka White | 2:51 | |
| Diddie Wah Diddie | Blind Blake | 2:59 | |
| Dry Well Blues | Charley Patton | 3:19 | |
| Take A Walk With Me (Take2) | Robert Lockwood, Jr., JR. | 3:00 | |
| Dark Road Blues | Willie Lofton | 3:03 | |
| I'll Be Rested (When The Roll Is Called) | Blind Roosevelt Graves | 2:33 | |
| Old Dog Blue | Jim Jackson | 2:58 | |
| Down On Penny's Farm | Bentley Boys | 2:48 | |
| Sugar Baby - 2020 Remaster | Dock Boggs | 2:58 | |
| Catfish Blues | Robert Petway | 2:55 | |
| Boogie Woogie Dance | Tampa Red | 3:14 | |
| You're Gonna Need Somebody When You Die | Charley Patton | 2:55 | |
| Alabama Bound - Version; Take 2; Remastered | Mance Lipscomb | 2:36 | |
| Dig Myself a Hole | Arthur "Big Boy" Crudup | 2:23 | |
| Old Blue | Furry Lewis | 3:00 | |
| Goin' Away Baby - Single Version | Jimmy Rogers | 2:46 | |
| Baby Please Don't Go | Pink Anderson | 2:45 | |
| Levee Camp Moan | Forest City Joe | 5:19 | |
| I Am The Light Of This World | Rev. Gary Davis | 3:35 | |
| I've Got Love If You Want It | Slim Harpo | 2:46 | |
| Shake 'Em on Down (Version 2) | Bukka White | 3:40 | |
| Rollin' And Tumblin' | Big Joe Williams | 2:36 | |