| Black Angel Blues | Robert Nighthawk | 3:04 | |
| Mississippi Boweavil Blues | Charley Patton | 3:11 | |
| See That My Grave's Kept Clean | Blind Lemon Jefferson | 2:53 | |
| Sugar Baby - 2020 Remaster | Dock Boggs | 2:58 | |
| Cut You Loose | T-Model Ford | 3:25 | |
| Ain't You Sorry | Mance Lipscomb | 2:20 | |
| You Missed a Good Man | Tampa Red | 3:34 | |
| Fishing Blues | Henry Thomas | 2:42 | |
| I'll Be Rested (When The Roll Is Called) | Blind Roosevelt Graves | 2:33 | |
| Sitting On Top Of The World | Mississippi Sheiks | 3:12 | |
| Sugar Babe | Mance Lipscomb | 2:06 | |
| Sic 'Em Dogs On | Bukka White | 2:27 | |
| County Jail Blues | Big Maceo | 2:55 | |
| Canned Heat Blues | Tommy Johnson | 3:39 | |
| The Bower of Prayer | Various Artists, Alan Lomax | 1:29 | |
| Dark Was the Night, Cold Was the Ground | Blind Willie Johnson | 3:18 | |
| John Henry | Henry Thomas | 2:43 | |
| You Can't Get That Stuff No More | Tampa Red, Georgia Tom | 2:56 | |
| How Long | Frank Stokes | 3:18 | |
| Moses Was A Servant of the Lord | Charles Barnett, Alan Lomax | 1:55 | |
| The Banks of the Arkansas / Wave the Ocean | Neil Morris | 1:58 | |
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| Cocaine Habit Blues | Memphis Jug Band | 2:48 | |
| Old Country Stomp | Henry Thomas | 2:51 | |
| Sugar Mama Blues | Sonny Boy Williamson I | 2:58 | |
| Pushin' My Luck | Robert Belfour | 4:34 | |
| Present Joys | Various Artists, Alan Lomax | 1:59 | |
| I'll Do Anything You Say | Charles Caldwell | 2:59 | |
| The Old Ship of Zion - Previously Unreleased | Ishman Williams & the William Singers, Alan Lomax | 4:32 | |
| Screamin' and Hollerin' the Blues | Charley Patton | 3:05 | |
| Further Up on the Road | David "Honeyboy" Edwards | 4:20 | |
| Ride Em on Down | Robert Petway | 2:56 | |
| John Henry | W.D. Stewart (Bama), Benny Will Richardson (22), Unidentified prisoners, Alan Lomax | 3:49 | |
| Church, I'm Fully Saved Today | Blind Willie Johnson | 3:06 | |
| If You Like Fat Women | Cedell Davis | 2:09 | |
| Back Door Blues | Kokomo Arnold | 3:25 | |
| Hoodoo Lady | Memphis Minnie | 3:02 | |
| I Got to Cross the River Jordan | Blind Willie McTell | 4:38 | |
| Bull-Doze Blues (Alternate Take) | Henry Thomas | 3:27 | |
| Pig Meat Is What I Crave | Bo Carter | 2:51 | |
| 61 Highway | Mississippi Fred McDowell | 3:11 | |
| John Henry | Furry Lewis | 5:49 | |
| Baby Please Don't Go | Mississippi Fred McDowell | 4:48 | |
| Broke Down Engine Blues | Blind Willie McTell | 2:45 | |
| Tryin' To Get Home | Rev. Gary Davis | 3:47 | |
| Greasy Greens | Pink Anderson | 4:38 | |
| Evil Hearted Woman - Alternate Take | T-Bone Walker | 2:36 | |
| My Baby She Gonna Jump And Shout | Mississippi Fred McDowell | 2:40 | |
| A Thousand Miles From Nowhere | Robert Pete Williams | 5:14 | |
| Rollin' And Tumblin' | Big Joe Williams | 2:36 | |
