| Talking Banjo | Buzz Busby | 1:39 | |
| Banjo Pickin' Girl | Stringbean | 2:24 | |
| Mistreated Mama Blues | Dock Boggs | 1:54 | |
| The Banks of the Ohio | The Kossoy Sisters | 3:19 | |
| Bringing in the Georgia Mail | Alex Campbell, Ola Belle Reed, Burl Kilby | 2:03 | |
| Salt Creek | Clarence White | 2:39 | |
| Long Haired Preachers (Preacher and the Slave) | Harry McClintock | 3:42 | |
| Black Eye Susie | Roscoe Holcomb | 1:14 | |
| Fishing Blues | Henry Thomas | 2:42 | |
| Cripple Creek | Clarence White | 1:02 | |
| Ain't No Grave | The Crooked Jades | 3:26 | |
| Coal Miner's Blues - 2021 Remaster | Hazel Dickens, Alice Gerrard | 2:42 | |
| Big Cheeseburgers and Good French Fries | Blaze Foley | 2:29 | |
| Preacher and the Bear | David Reed | 1:03 | |
| Railroad Bill | Etta Baker, Cora Phillips | 1:33 | |
| Bo Lamkin | Frank Proffitt | 4:08 | |
| Troubles | Anna & Elizabeth | 2:14 | |
| Country Blues | Dock Boggs | 3:52 | |
| White House Blues | Charlie Poole | 3:31 | |
| My Home's Across the Blue Ridge Mountain | Ola Belle Reed, Alex Campbell, John Miller | 2:33 | |
| Little Maggie | Barbara Dane | 2:09 | |
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| Single Girl | The Kossoy Sisters | 2:16 | |
| Living The Right Life Now | Molly O'Day | 2:17 | |
| Touch the Sky - Club Mix | Ecto Cooler, David Reed | 6:09 | |
| Lazy John | Chance McCoy & The Appalachian String Band | 2:59 | |
| John Hardy | Frank Proffitt | 2:27 | |
| Pretty Saro | Rhiannon Giddens | 2:41 | |
| Take It Out On Me - MitiS Remix | Shift Four, MitiS, David Reed, Dirtyrock | 5:09 | |
| Foggy Mountain Top | Bill Monroe and Doc Watson | 2:46 | |
| Undone in Sorrow | Ola Belle Reed, Bud Reed, John Coffey | 3:20 | |
| Ida Red | Stringbean | 1:16 | |
| I'll Never Get Drunk No More | Frank Proffitt | 2:34 | |
| Little Pink | Chance McCoy & The Appalachian String Band | 5:02 | |
| The Little Glass Of Wine | Stanley Brothers | 3:05 | |
| That Train That Carried My Girl from Town | The Doc Watson Family | 2:20 | |
| Oh The Wind And Rain | Jody Stecher | 3:49 | |
| The Coo Coo Bird | Clarence Ashley | 2:56 | |
| Ola Belle's Blues | Ola Belle Reed, Kevin Roth | 2:22 | |
| Who's Gonna' Shoe Your Pretty Little Foot | Barbara Dane | 3:43 | |
| Lost Indian | Marion Sumner | 0:54 | |
| Moonshiner | Roscoe Holcomb | 2:00 | |
| Out on the Open West | Frank Fairfield | 3:13 | |
| Every Day Dirt | The Doc Watson Family | 2:08 | |
| And Am I Born To Die? | The Doc Watson Family | 3:31 | |
| Sugarfoot Rag | Bill Keith | 1:50 | |
| One-Dime Blues | Etta Baker | 2:35 | |
| Wildwood Flower | The New Lost City Ramblers, Mother Maybelle Carter | 3:49 | |
| God Put A Rainbow In The Clouds | Ola Belle Reed | 2:48 | |
| Skillet Good And Greasy | Dirk Powell, John Hermann, Tim O'Brien | 2:22 | |