| Constant Lowdown | Jody Stecher, Mike Seeger | 2:56 | |
| Kenny Wagner's Surrender | Mike Seeger | 2:51 | |
| Living The Right Life Now | Molly O'Day | 2:17 | |
| Say Old Man, Can You Play a Fiddle? | Eck Robertson, Tracy Schwarz, Mike Seeger | 2:53 | |
| Josh Thomas's Roustabout | Mike Seeger | 2:41 | |
| Gospel Plow | Chance McCoy & The Appalachian String Band | 4:37 | |
| Wake Up Jacob - 2020 Remaster | Prince Albert Hunt's Texas Ramblers | 2:55 | |
| Harper | Tom Paxton | 2:52 | |
| Around the World | Mike Seeger | 2:23 | |
| Girl of Constant Sorrow | Barbara Dane | 4:06 | |
| Down South Blues | Dock Boggs | 2:11 | |
| Suwannee River | Mary Z. Cox | 0:57 | |
| Soldier's Joy - Instrumental | Blaine Sprouse | 3:34 | |
| The Golden Vanity | Jody Stecher | 5:28 | |
| Old Mountain Dew | The Delmore Brothers | 2:34 | |
| Goodbye Old Paint | Harry McClintock | 2:49 | |
| Cocaine (Original Mix) | Dick Justice | 3:01 | |
| The Iowa Waltz | Greg Brown | 3:56 | |
| Bright Sunny South | Dock Boggs | 3:41 | |
| Sal's Got Mud Between Her Toes | The Gilded Bats | 3:04 | |
| Little Maggie | Barbara Dane | 2:09 | |
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| Oh Molly Dear | B.F. Shelton | 3:00 | |
| Engine 143 | The Kossoy Sisters | 4:17 | |
| Jay Gould's Daughter | Nora Brown | 3:56 | |
| Ira Hayes (Ballad of Ira Hayes) | Peter La Farge | 3:35 | |
| My Long Journey Home | The Monroe Brothers | 2:29 | |
| The Silver Dagger | Old Crow Medicine Show | 4:06 | |
| The Roseville Fair | Bill Staines | 3:32 | |
| Green Rocky Road | Paul Clayton | 1:46 | |
| Willie Moore | Burnett & Rutherford | 3:15 | |
| I Never Will Marry | The Kossoy Sisters | 2:48 | |
| Swannanoa Tunnel | Mary Z. Cox | 2:58 | |
| Pretty Saro | Tatiana Hargreaves, Mark Schatz | 4:25 | |
| Fat Boy | Blaze Foley | 2:58 | |
| Wreck of the Dandenong | Bruce Molsky | 4:36 | |
| Sally Goodin | Reeltime Travellers | 2:49 | |
| Davy Come Back And Act Like You Ought To | Chance McCoy & The Appalachian String Band | 2:57 | |
| When the Train Comes Along | Henry Thomas | 2:56 | |
| Your Long Journey | The Doc Watson Family | 2:36 | |
| Black-Eyed Susie | Uncle Earl | 1:49 | |
| Lonesome Moonlight Waltz | Kenny Baker | 2:47 | |
| Liberty Dance | Autoharp Workshop, Mike Seeger | 1:17 | |
| I'm Bound To Ride | Fiddlin' Arthur Smith, His Dixieliners | 2:19 | |
| Road To Columbus | Kenny Baker | 2:39 | |
| Dill Pickle Rag | Don Reno, Bill Harrell, The Tennessee Cut-Ups | 1:38 | |
| Working Girl Blues | Hazel Dickens, Alice Gerrard | 3:21 | |
| The Old Hen Cackled And The Rooster Laid The Egg | Mike Seeger, Penny Seeger | 1:35 | |
| Rambling Hobo | Gaither Carlton | 1:43 | |
| Nancy Whiskey | Ian & Sylvia | 2:37 | |