| Track Lining Song | John (Black Sampson) Gibson, Alan Lomax | 1:25 | |
| Morning Prayer | Various Artists, Alan Lomax | 2:33 | |
| I Crave My Pig Meat | Blind Boy Fuller | 2:51 | |
| Ain't No More Cane on the Brazos | Ernest "Mexico" Williams, James "Iron Head" Baker and his Quartet, Alan Lomax | 2:52 | |
| Poor Boy | Harry McClintock | 2:14 | |
| If I Had My Way | Rev. Gary Davis | 4:46 | |
| Little Birdie | The Kossoy Sisters | 2:56 | |
| Huntington | Various Artists, Alan Lomax | 2:37 | |
| John Hardy | Mike Seeger | 2:44 | |
| The Devil's Dream | Sid Hemphill | 3:09 | |
| Sam Bass | Alan Lomax | 3:27 | |
| Don't Sing Love Songs | Barbara Dane | 4:00 | |
| Rosie | Unidentified Mississippi State Penitentiary (Parchman Farm) prisoners, Alan Lomax | 2:55 | |
| Murder's Home | Alan Lomax | 0:50 | |
| Minglewood Blues | Cannon's Jug Stompers | 3:40 | |
| All Night Long - Remastered | Miles Pratcher, Bob Pratcher, Alan Lomax | 3:04 | |
| Jesus Is Real to Me | Mary Lee | 3:14 | |
| Take Your Burden to the Lord and Leave It There | Washington Phillips | 3:15 | |
| Graveyard Blues | Roscoe Holcomb | 3:01 | |
| Levee camp holler (Downtown Money Waster) - Remastered | Johnny Lee Moore, Alan Lomax | 2:50 | |
| So Soon I'll Be At Home | Sid Hemphill | 1:34 | |
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| Bull-Doze Blues (Alternate Take) | Henry Thomas | 3:27 | |
| Tom Dooley | Frank Proffitt | 2:44 | |
| Judge Harsh Blues | Furry Lewis | 3:05 | |
| What Will We Do with the Baby O | The Kossoy Sisters | 1:46 | |
| My Epitaph | Ola Belle Reed, Bud Reed, David Reed | 3:16 | |
| A Spoonful Blues | Charley Patton | 3:12 | |
| Moses Was A Servant of the Lord | Charles Barnett, Alan Lomax | 1:55 | |
| Jesus Is My Friend | Washington Phillips | 2:53 | |
| Future Blues | Willie Brown | 3:01 | |
| Leatherwing Bat | Peggy Seeger | 2:11 | |
| Come On Boys, Let's Go To the Ball | Alec Askew, Alan Lomax | 1:31 | |
| The Dying Cowboy | Cisco Houston | 3:03 | |
| Prettiest Train | Alan Lomax | 3:43 | |
| Train on the Island | Hazel Dickens, Alice Gerrard | 1:44 | |
| Mount Zion | Various Artists, Alan Lomax | 2:03 | |
| The Lone Star Trail | Ken Maynard | 3:12 | |
| Stewball | Unidentified Mississippi State Penitentiary (Oakley) prisoners, Alan Lomax | 4:10 | |
| Did You Ever See the Devil, Uncle Joe? - Remastered | Wade Ward, Charlie Higgins | 1:53 | |
| Paul And Silas In Jail | Washington Phillips | 2:41 | |
| Riding In A Buggy - Previously Unreleased | Vera Hall | 2:08 | |
| Bukka's Jitterbug Swing | Bukka White | 2:39 | |
| Keep My Skillet Good and Greasy | Sid Hemphill | 3:37 | |
| Lazarus - Remastered | Henry Morrison, Alan Lomax | 1:42 | |
| Free at Last | Dock Reed, Vera Hall | 1:41 | |
| Kassie Jones (Casey Jones) | Furry Lewis | 2:58 | |
| Lonesome Valley | The Fairfield Four | 4:07 | |
| I Am The Light Of This World | Rev. Gary Davis | 3:35 | |
| Tryin' To Get Home | Rev. Gary Davis | 3:47 | |