| Timber (Jerry the Mule) | Josh White | 2:10 | |
| Dry Southern Blues | Blind Lemon Jefferson | 2:53 | |
| High Water Everywhere, Pt. 1 | Charley Patton | 3:08 | |
| Joe Hill | Paul Robeson | 3:00 | |
| Trouble in Mind - Version; Take 1; Remastered | Mance Lipscomb | 3:09 | |
| Keep My Skillet Good and Greasy | Uncle Dave Macon | 2:32 | |
| Devil's Gonna Git You | Bessie Smith | 3:10 | |
| You Get Down by the Pool Hall Clickety Clack - Sister Song | Michael Hurley | 3:34 | |
| Last Kind Word Blues | Geeshie Wiley | 3:04 | |
| The Preacher and the Slave | Utah Phillips | 2:23 | |
| Sugar Babe | Mance Lipscomb | 2:06 | |
| Old Country Stomp | Henry Thomas | 2:51 | |
| Mississippi Boweavil Blues | Charley Patton | 3:11 | |
| Dancing with Tears in My Eyes | Lead Belly | 3:11 | |
| 'Tain't Nobody's Business If I Do, Pt. 1 | Frank Stokes | 3:16 | |
| See That My Grave's Kept Clean | Blind Lemon Jefferson | 2:53 | |
| Working Class Woman | Barbara Dane | 6:26 | |
| Graveyard Blues | Roscoe Holcomb | 3:01 | |
| Bourgeois Blues | Lead Belly | 3:22 | |
| When The Levee Breaks | Memphis Minnie, Kansas Joe McCoy | 3:12 | |
| This Land is Not Our Land | Utah Phillips | 1:17 | |
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| I Get' the Blues | Bo Carter | 2:55 | |
| John Hardy - Version 2 | Lead Belly | 3:14 | |
| That Train That Carried My Girl from Town | The Doc Watson Family | 2:20 | |
| Praise God I'm Satisfied | Blind Willie Johnson | 3:10 | |
| I Am a Man of Constant Sorrow | Roscoe Holcomb | 3:00 | |
| Let's Disagree | Leroy Carr | 2:56 | |
| Ommie Wise - 2020 Remaster | G. B. Grayson | 3:13 | |
| Cigarettes, Whiskey And Wild, Wild Women | The Big Three Trio, Willie Dixon | 3:00 | |
| Southbound | The Doc Watson Family | 2:40 | |
| Minglewood Blues | Cannon's Jug Stompers | 3:40 | |
| County Farm Blues | Son House | 2:12 | |
| Round And Round Hitlers Grave | The Almanac Singers | 2:51 | |
| Goin' Back South | C.W. Stoneking | 3:30 | |
| Railroad Blues | Sam McGee | 2:48 | |
| Blue Ghost Blues | Lonnie Johnson | 3:09 | |
| Alabama Jubilee | R. Crumb And His Cheap Suit Serenaders | 2:40 | |
| Poor Ellen Smith | The Kossoy Sisters | 2:28 | |
| Red River Blues | Henry Thomas | 3:04 | |
| Going to Germany | Cannon's Jug Stompers | 2:31 | |
| Forty Four | Howlin' Wolf | 2:50 | |
| Little Annie Mae | Big Joe Williams | 2:00 | |
| Pure Religion | Rev. Gary Davis | 2:57 | |
| Moanin' At Midnight | Howlin' Wolf | 2:56 | |
| Sitting On Top Of The World | Howlin' Wolf | 2:35 | |
| That Black Snake Moan | Blind Lemon Jefferson | 2:59 | |
| Satan, Your Kingdom Must Come Down | Frank Proffitt | 1:51 | |
| Change My Luck Blues | Blind Lemon Jefferson | 2:24 | |
| Little Boy Blue | Scrapper Blackwell | 3:07 | |