| If the River Was Whiskey | Charlie Poole | 3:10 | |
| Kassie Jones, Pt. 2 | Furry Lewis | 3:04 | |
| Long Lonesome Road | Peggy Seeger | 2:17 | |
| Old Rub Alcohol Blues | Dock Boggs | 3:10 | |
| Canned Heat Blues | Tommy Johnson | 3:39 | |
| Turn Your Radio On | The Blue Sky Boys, Bill Bolick, Earl Bolick | 1:49 | |
| I Crave My Pig Meat | Blind Boy Fuller | 2:51 | |
| Satan, Your Kingdom Must Come Down | Frank Proffitt | 2:11 | |
| Mr. McTell Got the Blues - 2003 Remastered | Blind Willie McTell | 2:17 | |
| I Am The Light | Rev. Gary Davis | 3:02 | |
| Keep My Skillet Good and Greasy | Uncle Dave Macon | 2:32 | |
| Snake Baked a Hoecake | Peggy Seeger | 1:06 | |
| Dry Bones - 2020 Remaster | Bascom Lamar Lunsford | 3:00 | |
| Prayer of Death, Pt. 1 | Charley Patton | 3:06 | |
| High on a Mountain | Ola Belle Reed | 5:30 | |
| Way Down The Old Plank Road | Uncle Dave Macon, Sam McGee | 2:58 | |
| Fishing Blues | Henry Thomas | 2:42 | |
| Let Your Light Shine on Me | Blind Willie Johnson | 3:08 | |
| Ground Hog | The Doc Watson Family | 2:21 | |
| Parchman Farm Blues | Bukka White | 2:42 | |
| Further Up on the Road | David "Honeyboy" Edwards | 4:20 | |
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| Cuba | Dock Boggs | 1:29 | |
| Tomorrow Gon Be Too Late | C.W. Stoneking | 3:05 | |
| Tallahatchie River Blues | Mattie Delaney | 2:48 | |
| I Am a Weary and a Lonesome Traveller | Barbara Dane and the Chambers Brothers | 4:17 | |
| On the Banks of the Ohio - From Paper Moon | The Blue Sky Boys | 3:20 | |
| Sugar Babe | Mance Lipscomb | 2:06 | |
| Catfish Blues | Robert Petway | 2:55 | |
| Sally, Where'd You Get Your Liquor From | Rev. Gary Davis | 1:56 | |
| Kissing in the Dark | Memphis Minnie | 2:31 | |
| Poor Man | Frank Proffitt | 3:02 | |
| Walk Right In | Cannon's Jug Stompers | 2:55 | |
| Banks of the Ohio | Bill Monroe and Doc Watson | 3:33 | |
| Breadline Blues | The New Lost City Ramblers | 2:37 | |
| Wake Up Jacob - 2020 Remaster | Prince Albert Hunt's Texas Ramblers | 2:55 | |
| Go Ahead, Buddy | Casey Bill Weldon, Kokomo Arnold | 2:44 | |
| Charmin' Betsy | Henry Thomas | 3:01 | |
| Good Luck Charm | C.W. Stoneking | 2:45 | |
| When I Die | The Doc Watson Family | 2:17 | |
| No Disappointment in Heaven | Dock Boggs | 2:35 | |
| Chevrolet | Lonnie Young, Ed Young | 4:04 | |
| Travelling Back Home | Johnny Shines | 3:58 | |
| The Road Is Rough and Rocky | Archie Edwards | 3:27 | |
| Rock About Saro Jane | Uncle Dave Macon | 3:23 | |
| Sugar Blues | Dock Boggs | 1:26 | |
| Railroad Bill | Blind Boy Paxton, NO | 4:47 | |
| Mole in the Ground | Bascom Lamar Lunsford | 3:03 | |
| Brown's Ferry Blues | The Delmore Brothers | 2:41 | |
| Mining Camp Blues | Hazel Dickens, Alice Gerrard | 3:25 | |