| Milwaukee Blues | Charlie Poole | 3:18 | |
| Tallahatchie River Blues | Mattie Delaney | 2:48 | |
| Bright Sunny South | Dock Boggs | 3:39 | |
| County Farm Blues | Son House | 2:12 | |
| Bull-Doze Blues (Alternate Take) | Henry Thomas | 3:27 | |
| Railroad Bill | Blind Boy Paxton, NO | 4:47 | |
| I'm Troubled | The Doc Watson Family | 2:42 | |
| Statesboro Blues | Blind Willie McTell | 2:32 | |
| You're Gonna Need Somebody on Your Bond | Blind Willie Johnson | 3:09 | |
| If I Had My Way | Rev. Gary Davis | 4:46 | |
| Wake Up Jacob - 2020 Remaster | Prince Albert Hunt's Texas Ramblers | 2:55 | |
| Step It up and Go | Blind Boy Fuller | 2:59 | |
| The Banks of the Ohio | The Kossoy Sisters | 3:19 | |
| Sitting On Top Of The World | Mississippi Sheiks | 3:12 | |
| Kassie Jones (Casey Jones) | Furry Lewis | 2:58 | |
| Sail Away Lady - 2020 Remaster | Uncle Bunt Stephens | 2:58 | |
| If I Lose, I Don't Care | The North Carolina Ramblers, Charlie Poole | 3:09 | |
| You Got to Die | Blind Willie McTell | 3:12 | |
| Rambling Hobo | The Doc Watson Family | 1:38 | |
| Camp Meeting Tonight On the Old Camp Ground | Missisipi John Hurt | 2:20 | |
| Guitar Rag | Sylvester Weaver | 3:06 | |
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| Cluck Old Hen | Frank Proffitt | 2:23 | |
| Take a Drink on Me | Charlie Poole | 3:17 | |
| Shake It and Break It (But Don't Let It Fall Mama) | Charley Patton | 3:09 | |
| Down South Blues | Dock Boggs | 2:11 | |
| The Banks of the Arkansas / Wave the Ocean | Neil Morris | 1:58 | |
| Rag, Mama, Rag | Blind Boy Fuller | 3:03 | |
| Ragged And Dirty | William Brown | 4:50 | |
| Ditty Boy Twang | Michael Hurley | 3:08 | |
| Georgia Buck (Georgie Buck)(Never Let a Woman Have Her Way) | Dink Roberts | 1:03 | |
| Me and my uncle | Mike Wilhelm | 3:19 | |
| When Can I Change My Clothes? | Bukka White | 2:59 | |
| When the Levee Breaks - Alternate Take | Memphis Minnie | 3:09 | |
| A Lazy Farmer Boy | Buster Carter & Preston Young | 3:01 | |
| New Prisoner's Song | Dock Boggs | 2:57 | |
| The Butchers Boy - 2020 Remaster | Buell Kazee | 3:04 | |
| Screamin' and Hollerin' the Blues | Charley Patton | 3:05 | |
| John Henry | Furry Lewis | 4:51 | |
| The Death of Jerry Damron | Dock Boggs | 4:17 | |
| You Get Down by the Pool Hall Clickety Clack - Sister Song | Michael Hurley | 3:34 | |
| Evening Sun | Johnny Shines | 2:32 | |
| Cumberland Gap | Frank Proffitt | 1:25 | |
| Free Little Bird | Cousin Emmy, The New Lost City Ramblers | 1:00 | |
| Slow Drag (Cincinnati Flow Rag) - Instrumental | Rev. Gary Davis | 2:24 | |
| She-Wolf | Jessie Mae Hemphill | 4:41 | |
| Moanin' At Midnight | Howlin' Wolf | 2:56 | |
| Candy Man - Instrumental | Rev. Gary Davis | 2:52 | |
| Jack O' Diamonds Blues | Blind Lemon Jefferson | 2:44 | |
| Fly Around My Pretty Little Miss | Ola Belle Reed | 2:00 | |
