| I Got to Cross the River Jordan | Blind Willie McTell | 4:38 | |
| Nobody's Fault But Mine | Johnny Shines | 4:45 | |
| My Epitaph | Ola Belle Reed, Bud Reed, David Reed | 3:16 | |
| Little Birdie | The Kossoy Sisters | 2:56 | |
| Captain, Captain - Version; Take 2; Remastered | Mance Lipscomb | 2:34 | |
| If I Had My Way I'd Tear the Building Down | Blind Willie Johnson | 3:07 | |
| Cindy | Frank Proffitt | 2:16 | |
| Travelin' Higway Man | Tommy McClennan | 2:59 | |
| My Old Horse Died | Dock Boggs | 1:47 | |
| Don't Sing Love Songs | Barbara Dane | 4:00 | |
| Kissing in the Dark | Memphis Minnie | 2:31 | |
| Dry Bones - 2020 Remaster | Bascom Lamar Lunsford | 3:00 | |
| C.C. & O. Blues | Simmie Dooley, Pink Anderson | 3:05 | |
| Keep My Skillet Good and Greasy | Uncle Dave Macon | 2:32 | |
| The Butchers Boy - 2020 Remaster | Buell Kazee | 3:04 | |
| Bowling Green | The New Lost City Ramblers, Cousin Emmy | 1:50 | |
| Bottleneck Blues | Sylvester Weaver | 2:56 | |
| Old Rub Alcohol Blues | Dock Boggs | 3:10 | |
| Parchman Farm Blues | Bukka White | 2:42 | |
| Tomorrow Gon Be Too Late | C.W. Stoneking | 3:05 | |
| Old Dog Blue | Jim Jackson | 2:58 | |
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| Match Box Blues | Blind Lemon Jefferson | 3:03 | |
| Mashing That Thing | Bo Carter | 2:40 | |
| I Crave My Pig Meat | Blind Boy Fuller | 2:51 | |
| Wake Up Jacob - 2020 Remaster | Prince Albert Hunt's Texas Ramblers | 2:55 | |
| Loving Nancy | Dock Boggs | 2:30 | |
| Rabbit Chase | The New Lost City Ramblers | 2:36 | |
| Shady Grove | The Doc Watson Family | 2:17 | |
| Black Snake Moan | Blind Lemon Jefferson | 2:50 | |
| Jesus Make Up My Dying Bed | Blind Willie Johnson | 3:10 | |
| Sally, Where'd You Get Your Liquor From | Rev. Gary Davis | 1:56 | |
| Whoopee Blues | King Solomon Hill | 3:11 | |
| Statesboro Blues | Blind Willie McTell | 2:32 | |
| Alabama Jubilee | R. Crumb And His Cheap Suit Serenaders | 2:40 | |
| Rowan County Crew | Dock Boggs | 6:15 | |
| Banjo Tune | Erik Darling, The Kossoy Sisters, Robin Roberts | 1:42 | |
| Fixin' to Die Blues | Bukka White | 2:48 | |
| Look Down That Lonesome Road | The Doc Watson Family | 2:05 | |
| Minglewood Blues | Cannon's Jug Stompers | 3:40 | |
| Tom Dooley | Frank Proffitt | 2:44 | |
| What Will We Do with the Baby O | The Kossoy Sisters | 1:46 | |
| Blues on the Ceiling | Fred Neil | 2:24 | |
| Bob McKinney | Henry Thomas | 2:53 | |
| The World, She's Windin' Up | Cora Fluker, Ola Mae Bell | 2:51 | |
| Big Railroad Blues | Cannon's Jug Stompers | 3:18 | |
| Hesitation Blues | The Holy Modal Rounders | 2:19 | |
| Tryin' To Get Home | Rev. Gary Davis | 3:47 | |
| Rollin' And Tumblin' | Big Joe Williams | 2:36 | |
| Crawdad Hole | Jessie Mae Hemphill | 2:07 | |