| Memphis Rounders Blues | Frank Stokes | 2:51 | |
| Mashing That Thing | Bo Carter | 2:40 | |
| Mississippi County Farm Blues | Son House | 2:50 | |
| Henry Lee (Original Mix) | Dick Justice | 3:25 | |
| You're Gonna Need Somebody on Your Bond | Blind Willie Johnson | 3:09 | |
| Wagoner's Lad | Buell Kazee | 3:11 | |
| Kassie Jones (Casey Jones) | Furry Lewis | 2:58 | |
| Parchman Farm Blues | Bukka White | 2:42 | |
| Black Snake Moan | Blind Lemon Jefferson | 2:50 | |
| Sitting On Top Of The World | Mississippi Sheiks | 3:05 | |
| Cluck Old Hen | Frank Proffitt | 2:23 | |
| Sugar Babe | Mance Lipscomb | 2:06 | |
| C.C. & O. Blues | Simmie Dooley, Pink Anderson | 3:05 | |
| Trouble on My Mind (1888 Luscomb Banjo) | Clifton Hicks | 2:27 | |
| Canned Heat Blues | Tommy Johnson | 3:39 | |
| Turkey Reveille | Barbara Dane | 4:45 | |
| Look Down That Lonesome Road | The Doc Watson Family | 2:05 | |
| Parchman Farm Blues | Bukka White | 2:39 | |
| Rowdy Blues | Kid Bailey | 3:00 | |
| Little Maggie | Barbara Dane | 2:09 | |
| Wake Up Jacob - 2020 Remaster | Prince Albert Hunt's Texas Ramblers | 2:55 | |
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| Sic 'Em Dogs On | Bukka White | 2:27 | |
| Mistreated Mama Blues | Dock Boggs | 1:54 | |
| Cool Drink of Water Blues | Tommy Johnson | 3:27 | |
| Jennie Jenkins | Estil C. Ball & Orna Ball | 2:42 | |
| The Banks of the Arkansas / Wave the Ocean | Neil Morris | 1:58 | |
| Calvary | Dock Boggs | 3:33 | |
| Graveyard Blues | Roscoe Holcomb | 3:01 | |
| I'm A Bad Luck Woman | Memphis Minnie | 3:00 | |
| Terraplane Blues | Robert Johnson | 3:01 | |
| Statesboro Blues | Blind Willie McTell | 2:32 | |
| If I Had My Way I'd Tear the Building Down | Blind Willie Johnson | 3:07 | |
| Out on the Open West | Frank Fairfield | 3:13 | |
| Judge Harsh Blues | Furry Lewis | 3:05 | |
| Satan, Your Kingdom Must Come Down | Frank Proffitt | 2:11 | |
| Hiram Hubbard | Jean Ritchie and Doc Watson | 3:50 | |
| Bob McKinney | Henry Thomas | 2:53 | |
| 32-20 Blues | Robert Johnson | 2:47 | |
| Railroad Blues | Sam McGee | 2:48 | |
| Tom Dooley | Frank Proffitt | 2:44 | |
| I Got Mine | Frank Stokes | 3:06 | |
| If I Lose, I Don't Care | The North Carolina Ramblers, Charlie Poole | 3:09 | |
| Wayfaring Pilgrim | Ola Belle Reed | 4:33 | |
| Old Grey Mare | Norman Blake | 3:31 | |
| Hey, Hey Baby | The Holy Modal Rounders | 1:20 | |
| Free Little Bird | Cousin Emmy, The New Lost City Ramblers | 1:00 | |
| Sammie, Where Have You Been So Long | Dock Boggs | 3:05 | |
| Satan, Your Kingdom Must Come Down | Frank Proffitt | 1:51 | |
| Pure Religion | Rev. Gary Davis | 2:57 | |