| I've Got Blood In My Eyes For You | Mississippi Sheiks | 3:14 | |
| Every Day Dirt | The Doc Watson Family | 2:08 | |
| Big Road Blues | Tommy Johnson | 3:12 | |
| I Got Mine | Frank Stokes | 3:06 | |
| Down the Dirt Road Blues | Charley Patton | 2:54 | |
| The Road Is Rough and Rocky | Archie Edwards | 3:27 | |
| Willie Moore | The Kossoy Sisters | 3:05 | |
| Down On Penny's Farm | Bentley Boys | 2:48 | |
| Minglewood Blues | Cannon's Jug Stompers | 3:40 | |
| I Wish I Was a Mole in the Ground | Bascom Lamar Lunsford | 3:22 | |
| Kassie Jones (Casey Jones) | Furry Lewis | 2:58 | |
| Graveyard Blues | Roscoe Holcomb | 3:01 | |
| The Banks of the Ohio | The Kossoy Sisters | 3:19 | |
| Mole in the Ground | Bascom Lamar Lunsford | 3:03 | |
| The Butchers Boy - 2020 Remaster | Buell Kazee | 3:04 | |
| Canned Heat Blues | Tommy Johnson | 3:39 | |
| Last Kind Words Blues (Alternate Take) | Geeshie Wiley | 3:02 | |
| So Different Blues | Mance Lipscomb | 3:05 | |
| Old Joe's Barroom | Dock Boggs | 2:49 | |
| Turn Your Radio On (I) | John Hartford | 1:22 | |
| Undone in Sorrow | Ola Belle Reed, Bud Reed, John Coffey | 3:20 | |
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| Chevrolet | Lonnie Young, Ed Young | 4:04 | |
| Fallin' Down Blues | Robert Wilkins | 2:38 | |
| Mixed Blues | Dock Boggs | 3:55 | |
| Rope Stretching Blues | Blind Blake | 3:02 | |
| Old Mountain Dew | The Delmore Brothers | 2:34 | |
| Devil Sent the Rain Blues | Charley Patton | 3:05 | |
| Mississippi Delta Blues | Leon Redbone | 1:45 | |
| Buck Dance - Version; Instrumental; Remastered | Mance Lipscomb | 1:33 | |
| I'm So Glad You Don't Know What's On My Mind (take 1) | Jessie Mae Hemphill, Hezekiah, The Houserockers | 4:19 | |
| C.C. & O. Blues | Simmie Dooley, Pink Anderson | 3:05 | |
| Black Snake Moan | Blind Lemon Jefferson | 2:50 | |
| Franklin Roosevelt's Back Again | The New Lost City Ramblers | 2:21 | |
| Laughing to Keep from Crying | Mississippi Joe Callicott | 3:44 | |
| Say No to the Devil | Rev. Gary Davis | 3:20 | |
| Pony Blues | Charley Patton | 2:59 | |
| Ain't You Sorry | Mance Lipscomb | 2:20 | |
| Sail Away Lady - 2020 Remaster | Uncle Bunt Stephens | 2:58 | |
| Banjo Tune | Erik Darling, The Kossoy Sisters, Robin Roberts | 1:42 | |
| Don't Sing Love Songs | Barbara Dane | 4:00 | |
| Wild Bill Jones | Frank Proffitt | 3:27 | |
| Sleep Baby, Sleep | Jimmie Rodgers | 3:00 | |
| Mother's Advice | Dock Boggs | 3:42 | |
| Rag, Mama, Rag | Blind Boy Fuller | 3:03 | |
| La Le Too Dum | Barbara Dane | 3:18 | |
| Come On In My Kitchen | Robert Johnson | 2:52 | |
| Rambling Hobo | Gaither Carlton | 1:43 | |
| Aberdeen Mississippi Blues | Bukka White | 4:10 | |
| Pure Religion | Rev. Gary Davis | 2:57 | |