| A Spoonful Blues | Charley Patton | 3:12 | |
| How Come You're Being So Good to Me | John Hartford | 2:02 | |
| Rabbit Chase | The New Lost City Ramblers | 2:36 | |
| The Darby Ram | The Kossoy Sisters | 2:54 | |
| Guitar Rag | Sylvester Weaver | 3:06 | |
| Mississippi Delta Blues | Jimmie Rodgers | 3:25 | |
| Look Down That Lonesome Road | The Doc Watson Family | 2:05 | |
| Church, I'm Fully Saved Today | Blind Willie Johnson | 3:06 | |
| The Butchers Boy - 2020 Remaster | Buell Kazee | 3:04 | |
| Camp Meeting Tonight On the Old Camp Ground | Missisipi John Hurt | 2:20 | |
| Call Me A Dog When I'm Gone | Frank Fairfield | 2:24 | |
| One Meat Ball | Josh White | 3:01 | |
| That Train That Carried My Girl from Town | The Doc Watson Family | 2:20 | |
| Lone Prairie | Wade Ward | 0:56 | |
| Fine Artiste Blues | R. Crumb And His Cheap Suit Serenaders | 3:06 | |
| Worried Now, Won't Be Worried Long - Previously Unreleased | Sidney Carter, Alan Lomax | 2:55 | |
| The Lone Star Trail | Ken Maynard | 3:12 | |
| Eadie | Alan Lomax | 3:54 | |
| Darling Corey | The Doc Watson Family | 2:37 | |
| Banks of the Ohio | Bill Monroe and Doc Watson | 3:33 | |
| Little Birdie | The Kossoy Sisters | 2:56 | |
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| Parchman Farm Blues | Bukka White | 2:39 | |
| I'll Be Rested (When The Roll Is Called) | Blind Roosevelt Graves | 2:33 | |
| White House Blues | Charlie Poole | 3:31 | |
| Mule Skinner Blues | Bill Monroe & His Blue Grass Boys | 2:45 | |
| Sweet England | Shirley Collins | 2:35 | |
| Peg and Awl | Carolina Tar Heels | 2:57 | |
| It Makes a Long Time Man Feel Bad | Alan Lomax | 2:40 | |
| Minglewood Blues | Cannon's Jug Stompers | 3:40 | |
| The Arizona Yodeler | The DeZurik Sisters | 2:25 | |
| Your Long Journey | The Doc Watson Family | 2:36 | |
| Billy Lyons and Stack O'lee | Furry Lewis | 2:31 | |
| Blues Stay Away from Me | The Delmore Brothers | 2:49 | |
| Bright Sunny South | Dock Boggs | 3:41 | |
| She Thinks I Still Care | Harry Dean Stanton | 3:12 | |
| Banjo Tune | Erik Darling, The Kossoy Sisters, Robin Roberts | 1:42 | |
| Wake Up Jacob - 2020 Remaster | Prince Albert Hunt's Texas Ramblers | 2:55 | |
| Blue Mountain | Michael Hurley | 6:20 | |
| Rudee Down in New Orleans | Blaze Foley | 2:07 | |
| Train on the Island | Hazel Dickens, Alice Gerrard | 1:44 | |
| Going to Germany | Cannon's Jug Stompers | 2:31 | |
| I Got to Cross the River Jordan | Blind Willie McTell | 4:38 | |
| Cumberland Gap | Frank Proffitt | 1:25 | |
| Cod'ine | Buffy Sainte-Marie | 5:07 | |
| Free Little Bird | Cousin Emmy, The New Lost City Ramblers | 1:00 | |
| Don't Leave Your Records in the Sun | John Hartford | 2:26 | |
| Are You Afraid To Die | The Louvin Brothers | 2:35 | |
| Pack Up Your Sorrows | Mimi And Richard Farina | 3:02 | |
| Blues In The Bottle | The Holy Modal Rounders | 3:24 | |