| On the Road Again | Memphis Jug Band | 2:50 | |
| Diggin' My Potatoes | Washboard Sam | 2:58 | |
| Let Me Play With Your Noodle - Remastered | Tampa Red | 2:36 | |
| R.M. Blues | Roy Milton | 2:59 | |
| My Pencil Won't Write No More | Bo Carter | 2:57 | |
| Little Laura Blues | Sleepy John Estes | 2:31 | |
| See See Rider Blues | Ma Rainey | 3:15 | |
| Memories of My Trip | Brownie McGhee | 2:57 | |
| W.P.A. Blues | Casey Bill Weldon | 3:17 | |
| Canned Heat Blues | Tommy Johnson | 3:39 | |
| Stewball | Memphis Slim and Willie Dixon | 3:36 | |
| County Jail Blues | Big Maceo | 2:55 | |
| Evening Sun | Johnny Shines | 2:32 | |
| When Death Come Creeping in Your Room (Run, Sinner, Run) - Take 1; Remastered Version | Mance Lipscomb | 2:57 | |
| Guitar Rag | Sylvester Weaver | 3:06 | |
| Black Snake Swing | Victoria Spivey | 3:04 | |
| I Ain't Got You | Jimmy Reed | 2:15 | |
| Better Day | Brownie McGhee, Sonny Terry | 3:03 | |
| Why Don't You Eat Where You Slept Last Night? | Zuzu Bollin | 2:54 | |
| John the Revelator | Son House | 2:29 | |
| Black Train Blues | Bukka White | 2:56 | |
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| Homesick and Lonesome Blues | Blind Boy Fuller | 3:09 | |
| Beale St. Blues | Ottilie Patterson, Chris Barber's Jazz & Blues Band | 2:15 | |
| Three Songs - A Look at the Ragtime Era (Sister Kate's Night Out): I Wish I Could Shimmy Like My Sister Kate / Heebie Jeebies / 15 Cents | Jim Kweskin, Mel Lyman, Fritz Richmond | 3:22 | |
| Mississippi Heavy Water Blues | Barbecue Bob | 3:09 | |
| Cocaine Habit Blues | Memphis Jug Band | 2:48 | |
| I Crave My Pig Meat | Blind Boy Fuller | 2:51 | |
| Raise the Window High | Lonnie Johnson | 2:39 | |
| Lordy Lord It Hurts so Bad | Ottilie Patterson, Chris Barber's Jazz Band | 2:16 | |
| Step It up and Go | Blind Boy Fuller | 2:59 | |
| Mean Old Train | Joe Louis Walker, Bruce Katz, Giles Robson | 3:03 | |
| You Can't Get Away | Brownie McGhee, Sonny Terry | 2:58 | |
| Kay Cee Rider | Chris Barber | 2:09 | |
| What Have I Done | Jimmy Rogers | 2:45 | |
| White Boy Lost In The Blues | Sonny Terry, Brownie McGhee | 5:07 | |
| Goodnight Irene | Mississippi John Hurt | 2:25 | |
| I'm Gonna Miss You (Like The Devil) - Single Version | Slim Harpo | 2:20 | |
| Please Mr. Lofton - Live | John Mayall & The Bluesbreakers, Eric Clapton, Chris Barber | 7:03 | |
| Wild Cat Blues | Chris Barber | 2:53 | |
| Let The Good Times Roll | Louis Jordan | 2:42 | |
| Black Snake Blues | Victoria Spivey | 4:07 | |
| What's I'm Gotcha | Chris Barber's Jazz & Blues Band, Ottilie Patterson | 9:55 | |
| Spann's Stomp | Otis Spann's South Side Piano | 2:21 | |
| Red River Blues | Sonny Terry, Brownie McGhee | 3:19 | |
| There's Good Blues Tonight | Lucky Millinder Orchestra, Annisteen Allen | 2:28 | |
| Buzzin' | Slim Harpo | 2:05 | |
| Kill It Kid - Bonus Track | Blind Willie McTell | 2:31 | |
| St Philip Street Breakdown | Chris Barber's Jazz & Blues Band, Ottilie Patterson | 3:36 | |
| Mystery Train - Remastered 2022 | Little Junior Parker, The Blue Flames | 2:24 | |