| Catfish Blues | David "Honeyboy" Edwards | 3:30 | |
| Empire State Express | Son House | 3:39 | |
| Down the Dirt Road Blues | Charley Patton | 2:54 | |
| Let Me Play with Your Poodle | Tampa Red | 2:39 | |
| See That My Grave's Kept Clean | Blind Lemon Jefferson | 2:53 | |
| Night Time Is the Right Time - Take 1; Remastered Version | Mance Lipscomb | 4:49 | |
| Mashing That Thing | Bo Carter | 2:40 | |
| Cool Drink Of Water | Houston Stackhouse | 3:16 | |
| Hangman | Spider John Koerner, Dave Ray, Tony Glover | 2:32 | |
| Cocaine Habit Blues | Memphis Jug Band | 2:48 | |
| I Am a Weary and a Lonesome Traveller | Barbara Dane and the Chambers Brothers | 4:17 | |
| Mean Old Twister | Kokomo Arnold | 2:59 | |
| Cocaine (Original Mix) | Dick Justice | 3:01 | |
| Walk On | Sonny Terry and Brownie McGhee | 3:11 | |
| Sittin' On Top of the World | Sam Chatmon | 3:00 | |
| Wonderin' And Worryin' | Slim Harpo | 2:08 | |
| Parchman Farm Blues | Bukka White | 2:42 | |
| I'm a Rattlesnakin' Daddy | Blind Boy Fuller | 3:04 | |
| Which-A-Way Do Red River Run - Take 2; Remastered Version | Mance Lipscomb | 2:38 | |
| Cottonfield Blues, Pt. 2 | Garfield Akers | 3:21 | |
| I Will Turn Your Money Green (Alternate Take) | Furry Lewis | 3:11 | |
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| Alabama March | J.B. Lenoir | 2:53 | |
| Fixin' to Die Blues | Bukka White | 2:51 | |
| Fishing Blues | Henry Thomas | 2:42 | |
| Cross And Evil Woman Blues | Rev. Gary Davis | 3:02 | |
| We Gon' Boogaloo | C.W. Stoneking | 3:27 | |
| Dead Letter Blues | Son House | 3:31 | |
| Pussy Cat Blues | Bo Carter | 2:54 | |
| Baby You Gotta Change Your Mind | Blind Boy Fuller | 3:12 | |
| Bob McKinney | Henry Thomas | 2:53 | |
| Cotton Crop Blues | James Cotton | 3:01 | |
| Drop Down Mama | Sleepy John Estes | 3:11 | |
| You Can't Get That Stuff No More | Tampa Red, Georgia Tom | 2:56 | |
| Beefsteak Blues | James "Son" Thomas | 2:55 | |
| Mississippi Heavy Water Blues | Roscoe Holcomb | 2:17 | |
| Guitar Rag | Sylvester Weaver | 3:06 | |
| Ain't You Sorry | Mance Lipscomb | 2:20 | |
| That Train That Carried My Girl from Town | The Doc Watson Family | 2:20 | |
| Big Road Blues | Tommy Johnson | 3:12 | |
| Down On Penny's Farm | Bentley Boys | 2:48 | |
| Travelin' Higway Man | Tommy McClennan | 2:59 | |
| Baby Please Don't Go | Big Joe Williams | 2:44 | |
| Kissing in the Dark | Memphis Minnie | 2:31 | |
| Whoopee Blues | King Solomon Hill | 3:11 | |
| Death Valley Blues | Arthur "Big Boy" Crudup | 2:43 | |
| The Sinking Of The Titanic | Mance Lipscomb | 2:31 | |
| By The Spoonful | J.D. Short | 3:49 | |
| Goin' To Sit Down On The Banks Of The River | Rev. Gary Davis | 2:55 | |
| Mama Talk To Your Daughter | J.B. Lenoir | 2:21 | |