| Old Dog Blue | Jim Jackson | 2:58 | |
| See That My Grave's Kept Clean | Blind Lemon Jefferson | 2:54 | |
| Cocaine Habit Blues | Memphis Jug Band | 2:48 | |
| Little Laura Blues | Sleepy John Estes | 2:31 | |
| When The Levee Breaks | Memphis Minnie & Kansas Joe | 3:08 | |
| Bob McKinney | Henry Thomas | 2:53 | |
| Further Up on the Road | David "Honeyboy" Edwards | 4:20 | |
| Seminole Blues | Tampa Red | 3:05 | |
| Billy Lyons and Stack O'lee | Furry Lewis | 2:31 | |
| Levee Camp Blues | Son House | 3:45 | |
| Cocaine Done Kill My Baby | Mance Lipscomb | 2:33 | |
| Go Ahead, Buddy | Casey Bill Weldon, Kokomo Arnold | 2:44 | |
| Future Blues | Willie Brown | 3:01 | |
| Down By The Riverside | Sonny Terry and Brownie McGhee | 3:16 | |
| Walking Blues | Son House, Willie Brown, Fiddlin’ Joe Martin, Leroy Williams | 6:35 | |
| Kissing in the Dark | Memphis Minnie | 2:31 | |
| Black Angel Blues | Robert Nighthawk | 3:04 | |
| Let's Get Drunk Again | Sam Chatmon | 2:18 | |
| It's Nobody's Fault but Mine | Blind Willie Johnson | 3:08 | |
| Junker's Blues | Champion Jack Dupree | 3:12 | |
| You Can't Get That Stuff No More | Tampa Red | 2:52 | |
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| Bull-Doze Blues (Alternate Take) | Henry Thomas | 3:27 | |
| Pearline | Son House | 4:31 | |
| Minglewood Blues | Cannon's Jug Stompers | 3:40 | |
| The Prodigal Son | Robert Wilkins | 10:00 | |
| Joe Turner Killed a Man | Mance Lipscomb | 4:34 | |
| Nobody Knows You When Your Down and Out | Josh White | 3:00 | |
| Red River Blues | Henry Thomas | 3:04 | |
| Nobody's Fault But Mine | Johnny Shines | 4:45 | |
| God Don't Ever Change | Corey Harris | 2:10 | |
| You Was Born to Die | Blind Willie McTell | 2:51 | |
| Black Snake Moan | Blind Lemon Jefferson | 2:50 | |
| Baby You Gotta Change Your Mind | Blind Boy Fuller | 3:12 | |
| Down in Mississippi | J.B. Lenoir | 3:16 | |
| Alabama Bound - Version; Take 2; Remastered | Mance Lipscomb | 2:36 | |
| Shake 'Em On Down | Bukka White | 3:00 | |
| Black Cat Blues | Memphis Minnie | 2:54 | |
| Hell Hound On My Trail | Robert Johnson | 2:37 | |
| Catfish Blues | David "Honeyboy" Edwards | 3:30 | |
| Memphis Rounders Blues | Frank Stokes | 2:51 | |
| Fish Ain't Bitin' | Corey Harris | 3:28 | |
| High Water Everywhere, Pt. 1 | Charley Patton | 3:08 | |
| Sitting On Top Of The World | Mississippi Sheiks | 3:12 | |
| St. Louis Blues | Lonnie Johnson, Elmer Snowden | 3:05 | |
| Standing At The Crossroads | Johnny Shines | 2:54 | |
| Crawdad Hole | Jessie Mae Hemphill | 2:07 | |
| The Sinking Of The Titanic | Mance Lipscomb | 2:31 | |
| I Am The Light Of This World | Rev. Gary Davis | 3:35 | |
| Empire State Express | Son House | 4:30 | |