| Junkrat Vs Demoman Rap Battle | JT Music | 4:31 | |
| SDGAF | Jon Tron, The Gregory Brothers | 2:44 | |
| Mitosis vs Meiosis Rap Battle | AsapSCIENCE | 2:32 | |
| Life Is Fun | Boyinaband, TheOdd1sOut | 3:41 | |
| Jellyfish | Julian Smith | 1:22 | |
| Lord of the Rings in 99 Seconds | Jon Cozart | 1:39 | |
| Release the Kraken | Ninja Sex Party | 3:19 | |
| Squirrel Rights | Rhett and Link | 3:04 | |
| The Bayeux Tapestry | Animaniacs | 2:21 | |
| Don't Wanna Know | Bo Burnham | 1:03 | |
| Bushes of Love | Bad Lip Reading | 4:44 | |
| I Like What I Like (Reprise) | Rhett and Link | 1:47 | |
| Medic! - A Team Fortress 2 Musical | Random Encounters | 4:11 | |
| "Get Bloody,"Daft Punk Get Lucky Happy Wheels Parody | Tobuscus, Toby Turner | 3:01 | |
| The Black Friday Song | Rusty Cage | 1:46 | |
| What Is My Life | The Gregory Brothers, Jacksepticeye | 3:47 | |
| The World Needs Heroes | The Yogscast | 2:56 | |
| Art of Darkness | The Stupendium | 4:28 | |
| Sombra Vs Spy Rap Battle | JT Music, Andrea Storm Kaden | 3:57 | |
| Carl Poppa (feat. Carl G.) | Bad Lip Reading, Carl G. | 4:38 | |
| Everyone I Don't Like Is Literally Hitler | Rusty Cage | 2:02 | |
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| Death Trap | Neil Patrick Harris, Grey DeLisle | 1:49 | |
| Politiclash | Jon Cozart | 4:44 | |
| GFY | Ninja Sex Party | 1:06 | |
| When You Make a Musical Promise | Daniel Thrasher | 2:00 | |
| Narwhals | Mr Weebl | 1:59 | |
| Third Act Misunderstanding | Kevin Temmer | 1:27 | |
| Phatdippin' | Rhett and Link, Iman Crosson, Tay Zonday | 2:37 | |
| All Star but It's 24 Cartoon Character Impressions | Brock Baker | 2:34 | |
| Shit | Bo Burnham | 1:18 | |
| Put a Banana in Your Ear | Jason Steele | 1:20 | |
| Wakko's America | Animaniacs | 1:54 | |
| Fly Me to the Moon (EDM Version) | Black Gryph0n, Baasik | 1:47 | |
| Five Nights at Freddy's: Night 1 (feat. Markiplier) | Random Encounters, Markiplier | 2:56 | |
| The Christmas Knife Game Song | Rusty Cage | 1:43 | |
| First Date | Ninja Sex Party | 2:44 | |
| Don't Mine At Night - Minecraft Parody | Brad Knauber | 3:37 | |
| Resident Enis Monster Gulch: So Awful at All | Random Encounters, Markiplier, Dodger | 3:29 | |
| I Know Him | Jonathan Groff | 1:37 | |
| Reboot It | Animaniacs | 2:04 | |
| Crasher-Vania | Starbomb | 2:23 | |
| Star Wars in 99 Seconds | Jon Cozart | 1:39 | |
| We're Doing a Sequel | The Muppets, Lady Gaga, Tony Bennett | 4:16 | |
| Big Nose | Daniel Thrasher | 2:04 | |
| Sunny Side Up Summer | Bob's Burgers, H. Jon Benjamin, John Roberts, Dan Mintz, Kristen Schaal, Eugene Mirman | 3:57 | |
| Interrogation Song | Ty Burrell, Sam Eagle, The Muppets | 3:20 | |
| I'm Number One | Constantine, Ricky Gervais | 2:29 | |
| Robot Riot | Love Händel | 3:16 | |
| Danny Elephant - From "Descendants: A Bad Lip Reading" | Bad Lip Reading | 2:37 | |
