| Undone in Sorrow | Ola Belle Reed, Bud Reed, John Coffey | 3:20 | |
| Cruel War | Iron Mountain String Band | 2:41 | |
| Fox Chase | Deford Bailey | 3:01 | |
| Living The Right Life Now | Molly O'Day | 2:17 | |
| Rose of Sharon | Bobby Fullbright, Charles Summer, J.C. Owens, Jimmy Pruitt, Nick Hallman, Paul Gregory, TC Foster, Ted Brackett, Vernon Riddle | 2:18 | |
| Fishing Blues | Henry Thomas | 2:42 | |
| Gospel Plow | Chance McCoy & The Appalachian String Band | 4:37 | |
| Blues Stay Away from Me | The Delmore Brothers | 2:49 | |
| New Dying Soldier | Nick Shoulders | 4:12 | |
| June Apple | Foghorn Stringband | 3:58 | |
| Hog of the Foresaken | Michael Hurley | 4:35 | |
| Dance All Night With a Bottle in Your Hand | Cousin Emmy, The New Lost City Ramblers | 1:45 | |
| Lonesome Moonlight Waltz | Bill Monroe and Doc Watson | 1:35 | |
| Spider In The Window - Instrumental | Blaine Sprouse | 3:02 | |
| Talking Banjo | Buzz Busby | 1:39 | |
| Green Valley Waltz | Nora Brown, Jackson Lynch | 2:49 | |
| Railroad Bill | Etta Baker, Cora Phillips | 1:33 | |
| No Hard Times | Jimmie Rodgers | 3:07 | |
| Riding on That Train 45 | The New Lost City Ramblers | 2:21 | |
| Jaybird March | Etta and Cora Baker | 1:50 | |
| Wednesday Night Waltz | Chance McCoy & The Appalachian String Band | 3:04 | |
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| Knife Guitar | Roscoe Holcomb | 1:17 | |
| Raleigh and Spencer/Dusty Miller | Tatiana Hargreaves, Danny Knicely, Emma Beaton, Mark Schatz | 4:05 | |
| Rueben's Train | Meredith Moon | 2:45 | |
| Cocaine Done Kill My Baby | Mance Lipscomb | 2:33 | |
| Away Out On the Mountain | Jimmie Rodgers | 3:17 | |
| Hills of Mexico | Nora Brown | 4:10 | |
| Down On Penny's Farm | Bentley Boys | 2:48 | |
| Hallelujah, I'm a Bum | Harry McClintock | 2:57 | |
| Get Thee Behind Me, Satan | The Almanac Singers | 2:36 | |
| The East Virginia Blues | The Carter Family | 2:45 | |
| Who's Gonna' Shoe Your Pretty Little Foot | Barbara Dane | 3:43 | |
| The Butchers Boy - 2020 Remaster | Buell Kazee | 3:04 | |
| Sugar Hill | Mary Z. Cox | 3:33 | |
| Shadow of the Moon | The Flatlanders | 2:52 | |
| Moonshiner | Roscoe Holcomb | 2:00 | |
| John Hardy | Mike Seeger | 2:44 | |
| Swannanoa Tunnel | Bascom Lamar Lunsford | 3:00 | |
| Salt Creek | Clarence White | 2:39 | |
| Barbara Allen | Bradley Kincaid | 2:32 | |
| Little Sadie | Bill Keith, Jim Rooney | 1:27 | |
| Satan, Your Kingdom Must Come Down | Frank Proffitt | 1:51 | |
| Pretty Polly | Raymond Fairchild | 1:34 | |
| Where Have All The Average People Gone - Single Version | Roger Miller | 3:01 | |
| Nancy Whiskey | Ian & Sylvia | 2:37 | |
| Beaumont Rag | Tony Trischka | 2:04 | |
| Things In Life | Don Stover | 3:07 | |
| Worried Man Blues | Don Reno, Bill Harrell, The Tennessee Cut-Ups | 2:07 | |
| Long Journey Home | The Kentucky Colonels | 1:57 | |